使用免疫荧光测定法检测猫后睾吸虫特异性IgG抗体。
Detecting Aelurostrongylus abstrusus-specific IgG antibody using an immunofluorescence assay.
作者信息
Briggs Kaitlyn R, Yaros Joseph P, Liotta Janice L, Lucio-Forster Araceli, Lee Alice C Y, Bowman Dwight D
机构信息
Department of Immunology and Microbiology, Cornell University College of Veterinary Medicine, Ithaca, NY, USA.
出版信息
J Feline Med Surg. 2013 Dec;15(12):1114-8. doi: 10.1177/1098612X13489977. Epub 2013 May 24.
Abstract
Diagnosis of feline lungworm, Aelurostrongylus abstrusus, is typically achieved by identifying larvae in feces following concentration through flotation or using the Baermann technique. This work presents observations on the usefulness of an indirect immunofluorescence antibody assay for detection of antibodies to this parasite in the sera of infected cats. Using first-stage larvae of A abstrusus and sera from both experimentally and naturally infected cats, it was determined that the test was fairly sensitive and did not cross-react with serum from an Ancylostoma braziliense (hookworm)-infected cat.
摘要
猫肺线虫（隐匿猫圆线虫）的诊断通常是通过漂浮法浓缩粪便或使用贝尔曼氏技术来鉴定其中的幼虫来实现的。本文介绍了关于间接免疫荧光抗体检测法在检测感染猫血清中该寄生虫抗体的实用性的观察结果。使用隐匿猫圆线虫的一期幼虫以及来自实验感染和自然感染猫的血清，确定该检测相当灵敏，且不会与感染巴西钩口线虫（钩虫）的猫的血清发生交叉反应。
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本文引用的文献
1
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2
Improved detection of canine Angiostrongylus vasorum infection using real-time PCR and indirect ELISA.实时 PCR 和间接 ELISA 提高犬血管圆线虫感染检测。
Parasitol Res. 2011 Dec;109(6):1577-83. doi: 10.1007/s00436-011-2414-4. Epub 2011 May 3.
3
Prevalence of fecal-borne parasites detected by centrifugal flotation in feline samples from two shelters in upstate New York.通过离心浮选法在纽约州北部两个收容所的猫样本中检测到的粪源性寄生虫的患病率。
J Feline Med Surg. 2011 Apr;13(4):300-3. doi: 10.1016/j.jfms.2010.12.013. Epub 2011 Feb 22.
4
Helminth parasites of the canine and feline respiratory tract.犬猫呼吸道的蠕虫寄生虫。
Vet Clin North Am Small Anim Pract. 2009 Nov;39(6):1109-26, vii. doi: 10.1016/j.cvsm.2009.06.006.
5
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6
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Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(4):286-7.
8
Immunofluorescence as a diagnostic procedure in lungworm disease of the cat.
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9
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