The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Bioresour Technol. 2013 Jul;140:286-91. doi: 10.1016/j.biortech.2013.04.091. Epub 2013 May 3.
A keratin-degrading strain Stenotrophomonas maltophilia BBE11-1 was grown in a 3-L batch fermenter containing wool waste as the main medium and cell growth rate was determined as the key factor to affect keratinase yield. Three strategies of temperature-shift procedure, two-stage DO control and fed-batch process were used to change growth rate. And a 62.2% improvement of keratinase yield was achieved. With the glucose fed-batch procedure in 30-L fermenter, keratinase production was significantly improved up to 117.7% (1728 U/ml) as compared with initial data (793.8 U/ml) in a 3-L fermenter and with much shortened fermentation time within 18 h. Significant structure changes and high levels of free amino acids from wool decomposition indicated the possible applications for wool waste management and fertilizer industry. The remarkable digestion of wool cuticle also suggested its potential utilization in textile industry.
从羊毛废料中筛选到一株角蛋白降解菌 Stenotrophomonas maltophilia BBE11-1，在 3L 发酵罐中进行分批发酵，以羊毛废料为主要培养基，考察细胞生长速率对角蛋白酶产量的影响。采用变温策略、两段式溶氧控制和补料分批发酵三种方法改变生长速率，角蛋白酶产量提高了 62.2%。在 30L 发酵罐中进行葡萄糖补料分批发酵，角蛋白酶产量比初始数据（793.8 U/ml）提高了 117.7%（1728 U/ml），发酵时间缩短至 18h。从羊毛分解得到的大量游离氨基酸和明显的结构变化表明，该酶在羊毛废料管理和肥料工业中有很好的应用前景。角蛋白的显著消化也表明其在纺织工业中有潜在的应用价值。
