Starý J, Hrodek O, Hausner P, Petráková A, Goetz P, Kreuger A
Department of Pediatrics, University Hospital Motol, Prague, Czechoslovakia.
Neoplasma. 1990;37(3):293-9.
The prognostic value of cellular DNA content measured by static cytophotometry was evaluated in 69 children with acute lymphoblastic leukemia (ALL) using the pretreatment distribution of the DNA content in blast cells of bone marrow and peripheral blood. The median follow-up of the whole group of patients was 45 months. Aneuploidy was detected in 71% of children, most of them showing a hyperdiploid content (DNA index greater than 1.05). The duration of complete remission was significantly longer in patients with distinct hyperdiploid DNA content (DNA index greater than 1.16) than in those with less hyperdiploid and diploid DNA content (DNA index less than 1.16). The results achieved by static cytophotometry were compared with flow cytometry analysis and with cytogenetic investigations of chromosomal abnormalities in leukemic cells. Higher correlation was found between flow cytometry and cytogenetics. Flow cytometry proved to be a more convenient method for detection of the DNA content in leukemic cells than static cytophotometry.
采用骨髓和外周血原始细胞DNA含量的预处理分布，对69例急性淋巴细胞白血病（ALL）患儿进行了静态细胞光度法测量细胞DNA含量的预后价值评估。全组患者的中位随访时间为45个月。71%的儿童检测到非整倍体，其中大多数表现为超二倍体含量（DNA指数大于1.05）。DNA含量明显超二倍体（DNA指数大于1.16）的患者完全缓解持续时间明显长于超二倍体和二倍体含量较低（DNA指数小于1.16）的患者。将静态细胞光度法的结果与流式细胞术分析以及白血病细胞染色体异常的细胞遗传学研究结果进行了比较。发现流式细胞术与细胞遗传学之间的相关性更高。流式细胞术被证明是一种比静态细胞光度法更方便的检测白血病细胞DNA含量的方法。
