School of Science, Monash University, Sunway Campus, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia.
Appl Biochem Biotechnol. 2013 Aug;170(7):1602-13. doi: 10.1007/s12010-013-0298-8. Epub 2013 May 26.
Oil palm empty fruit bunch (EFB) is abundantly available in Malaysia and it is a potential source of xylose for the production of high-value added products. This study aimed to optimize the hydrolysis of EFB using dilute sulfuric acid (H2SO4) and phosphoric acid (H3PO4) via response surface methodology for maximum xylose recovery. Hydrolysis was carried out in an autoclave. An optimum xylose yield of 91.2 % was obtained at 116 °C using 2.0 % (v/v) H2SO4, a solid/liquid ratio of 1:5 and a hydrolysis time of 20 min. A lower optimum xylose yield of 24.0 % was observed for dilute H3PO4 hydrolysis at 116 °C using 2.4 % (v/v) H3PO4, a solid/liquid ratio of 1:5 and a hydrolysis time of 20 min. The optimized hydrolysis conditions suggested that EFB hydrolysis by H2SO4 resulted in a higher xylose yield at a lower acid concentration as compared to H3PO4.
油棕空果串(EFB)在马来西亚大量存在,是生产高附加值产品的木糖潜在来源。本研究旨在通过响应面法优化稀硫酸(H2SO4)和磷酸(H3PO4)对 EFB 的水解,以获得最大的木糖收率。水解在高压釜中进行。在 116°C 下,使用 2.0%(v/v)H2SO4、固液比为 1:5 和水解时间为 20 min,可获得 91.2%的最佳木糖收率。在 116°C 下,使用 2.4%(v/v)H3PO4、固液比为 1:5 和水解时间为 20 min,稀 H3PO4 水解的最佳木糖收率较低,为 24.0%。优化的水解条件表明,与 H3PO4 相比,EFB 用 H2SO4 水解在较低的酸浓度下可获得更高的木糖收率。