利用 CRISPR RNA 指导的 Cas9 核酸酶对果蝇进行基因组工程改造。
Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.
机构信息
Genetics Training Program, University of Wisconsin, Madison, Madison, WI 53706, USA.
出版信息
Genetics. 2013 Aug;194(4):1029-35. doi: 10.1534/genetics.113.152710. Epub 2013 May 24.
Abstract
We have adapted a bacterial CRISPR RNA/Cas9 system to precisely engineer the Drosophila genome and report that Cas9-mediated genomic modifications are efficiently transmitted through the germline. This RNA-guided Cas9 system can be rapidly programmed to generate targeted alleles for probing gene function in Drosophila.
摘要
我们对细菌 CRISPR RNA/Cas9 系统进行了改造，以精确地对果蝇基因组进行工程改造，并报告 Cas9 介导的基因组修饰能够有效地通过生殖系传递。这种 RNA 引导的 Cas9 系统可以快速编程，以生成用于探测果蝇中基因功能的靶向等位基因。
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