A Toolkit of CRISPR-Based Genome Editing Systems in Drosophila.

作者信息

Xu Jiang, Ren Xingjie, Sun Jin, Wang Xia, Qiao Huan-Huan, Xu Bo-Wen, Liu Lu-Ping, Ni Jian-Quan

机构信息

Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China; School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.

Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.

出版信息

J Genet Genomics. 2015 Apr 20;42(4):141-9. doi: 10.1016/j.jgg.2015.02.007. Epub 2015 Mar 12.

DOI:10.1016/j.jgg.2015.02.007

PMID:25953352

Abstract

The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.

摘要