Xu Jiang, Ren Xingjie, Sun Jin, Wang Xia, Qiao Huan-Huan, Xu Bo-Wen, Liu Lu-Ping, Ni Jian-Quan
Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China; Tsinghua Fly Center, Tsinghua University, Beijing 100084, China; School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China; College of Bioengineering, Hubei University of Technology, Wuhan 430068, China.
Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing 100084, China.
J Genet Genomics. 2015 Apr 20;42(4):141-9. doi: 10.1016/j.jgg.2015.02.007. Epub 2015 Mar 12.
The last couple of years have witnessed an explosion in development of CRISPR-based genome editing technologies in cell lines as well as in model organisms. In this review, we focus on the applications of this popular system in Drosophila. We discuss the effectiveness of the CRISPR/Cas9 systems in terms of delivery, mutagenesis detection, parameters affecting efficiency, and off-target issues, with an emphasis on how to apply this powerful tool to characterize gene functions.
在过去几年中，基于CRISPR的基因组编辑技术在细胞系以及模式生物中的发展呈爆发式增长。在本综述中，我们重点关注这一流行系统在果蝇中的应用。我们从递送、诱变检测、影响效率的参数以及脱靶问题等方面讨论了CRISPR/Cas9系统的有效性，重点在于如何应用这一强大工具来阐明基因功能。
