Engineering Center of Ministry of Education for Standardization of Chinese Medicine Processing, Nanjing University of Chinese Medicine, Nanjing 210023, China ; Nanjing Haichang Chinese Medicine Group Co., Ltd., Nanjing 210061, China.
Evid Based Complement Alternat Med. 2013;2013:237541. doi: 10.1155/2013/237541. Epub 2013 Apr 28.
Pericarpium Citri Reticulatae (Chenpi in Chinese) has been widely used as an herbal medicine in Korea, China, and Japan. Chenpi extracts are used to treat indigestion and inflammatory syndromes of the respiratory tract such as bronchitis and asthma. This thesis will analyze chemical compositions of Chenpi volatile oil, which was performed by comprehensive two-dimensional gas chromatography with high-resolution time-of-flight mass spectrometry (GC × GC-HR-TOFMS). One hundred and sixty-seven components were tentatively identified, and terpene compounds are the main components of Chenpi volatile oil, a significant larger number than in previous studies. The majority of the eluted compounds, which were identified, were well separated as a result of high-resolution capability of the GC × GC method, which significantly reduces, the coelution. β -Elemene is tentatively qualified by means of GC × GC in tandem with high-resolution TOFMS detection, which plays an important role in enhancing the effects of many anticancer drugs and in reducing the side effects of chemotherapy. This study suggests that GC × GC-HR-TOFMS is suitable for routine characterization of chemical composition of volatile oil in herbal medicines.
陈皮（Chenpi）在中国、韩国和日本被广泛用作草药。陈皮提取物被用于治疗消化不良和呼吸道炎症，如支气管炎和哮喘。本论文将通过全二维气相色谱与高分辨率飞行时间质谱联用（GC×GC-HR-TOFMS）分析陈皮挥发油的化学成分。共鉴定出 167 种成分，萜类化合物是陈皮挥发油的主要成分，比以往的研究数量显著增加。由于 GC×GC 方法的高分辨率能力，大多数洗脱化合物得到了很好的分离，显著减少了共洗脱。β-榄香烯通过 GC×GC 串联高分辨率 TOFMS 检测被暂定定性，它在增强许多抗癌药物的疗效和降低化疗副作用方面发挥着重要作用。本研究表明，GC×GC-HR-TOFMS 适用于中草药挥发油化学成分的常规表征。
