Department of Horticulture, Agricultural Science Center North, University of Kentucky, Room 308J, Lexington, KY 40546, USA ; Seed Biology Group, University of Kentucky, Lexington, KY 40546, USA.
Comput Math Methods Med. 2013;2013:653759. doi: 10.1155/2013/653759. Epub 2013 Apr 28.
This review highlights discoveries made using phage display that impact the use of agricultural products. The contribution phage display made to our fundamental understanding of how various protective molecules serve to safeguard plants and seeds from herbivores and microbes is discussed. The utility of phage display for directed evolution of enzymes with enhanced capacities to degrade the complex polymers of the cell wall into molecules useful for biofuel production is surveyed. Food allergies are often directed against components of seeds; this review emphasizes how phage display has been employed to determine the seed component(s) contributing most to the allergenic reaction and how it has played a central role in novel approaches to mitigate patient response. Finally, an overview of the use of phage display in identifying the mature seed proteome protection and repair mechanisms is provided. The identification of specific classes of proteins preferentially bound by such protection and repair proteins leads to hypotheses concerning the importance of safeguarding the translational apparatus from damage during seed quiescence and environmental perturbations during germination. These examples, it is hoped, will spur the use of phage display in future plant science examining protein-ligand interactions.
本文综述了噬菌体展示技术在农产品应用方面的研究进展。讨论了噬菌体展示技术在深入了解各种保护分子如何保护植物和种子免受草食动物和微生物侵害方面的贡献。调查了噬菌体展示技术在定向进化酶方面的应用，这些酶具有增强的能力将细胞壁的复杂聚合物降解为生物燃料生产有用的分子。食物过敏通常针对种子的成分；本文强调了噬菌体展示如何用于确定对过敏反应贡献最大的种子成分，以及它在减轻患者反应的新方法中如何发挥核心作用。最后，概述了噬菌体展示在鉴定成熟种子蛋白质组保护和修复机制中的应用。鉴定出受此类保护和修复蛋白优先结合的特定蛋白类群，使人们提出了关于在种子休眠期间和萌发期间环境胁迫期间保护翻译装置免受损伤的重要性假设。希望这些例子能够激发未来植物科学中使用噬菌体展示技术来研究蛋白质-配体相互作用。
