Buranaamnuay K
Reproductive Biology Research Group, Insititute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Reprod Domest Anim. 2013 Dec;48(6):912-7. doi: 10.1111/rda.12185. Epub 2013 May 24.
Effects of sperm-TALP (TALP) on the quality of fresh-extended and frozen-thawed epididymal cat sperm were evaluated. The epididymides suspended in Tris-glucose-citrate solution (Tris), a conventional medium, and TALP were cut into small pieces to recover epididymal sperm. In experiment 1, the sperm pellets remained after centrifugation were re-suspended (1 : 2, v/v) in Tris and TALP. The sperm quality in all four groups, that is, sperm retrieved with Tris (I and II) or TALP (III and IV) and diluted with Tris (I and III) or TALP (II and IV) was assessed. The sperm motility at the 0-h incubation in TALP-TALP was superior to that of the rest (p < 0.001 to p = 0.04). At the 2-h incubation, the motility in Tris/TALP-TALP was greater than that in Tris/TALP-Tris (p ≤ 0.001). In experiment 2, after centrifugation, the sperm pellets were added with freezing extenders and frozen. The thawed sperm previously retrieved from the epididymides with Tris and TALP were allotted so as not to further diluted (Tris/TALP-O) and to further diluted (1 : 1, v/v) with Tris (Tris/TALP-Tris) and TALP (Tris/TALP-TALP) and were evaluated the quality. At both incubation times, the motility of frozen-thawed sperm recovered with TALP (TALP-O/Tris/TALP) was comparable with or significantly higher than that in the Tris groups (Tris-O/Tris/TALP; p = 0.003 to p > 0.05). The motility and viability of thawed sperm in Tris-Tris were significantly decreased during the 2-h incubation (p = 0.007 for the motility and p = 0.01 for the viability). In both experiments, neither type of diluent (Tris vs TALP) nor incubation time (0 vs 2 h) significantly affected the sperm membrane integrity under hypo-osmotic condition (p > 0.05). According to beneficial effects on the quality of fresh-extended and frozen-thawed sperm demonstrated, sperm-TALP could be used as an alternative medium for recovering sperm from the epididymides and for diluting epididymal sperm in the domestic cat.
评估了精子-输卵管液（TALP）对新鲜稀释和冻融附睾猫精子质量的影响。将悬浮于传统培养基Tris-葡萄糖-柠檬酸盐溶液（Tris）和TALP中的附睾切成小块以回收附睾精子。在实验1中，将离心后剩余的精子沉淀重悬（1:2，v/v）于Tris和TALP中。评估了所有四组的精子质量，即使用Tris（I和II）或TALP（III和IV）回收并用Tris（I和III）或TALP（II和IV）稀释的精子。在TALP-TALP中孵育0小时时精子活力优于其他组（p<0.001至p = 0.04）。在孵育2小时时，Tris/TALP-TALP中的活力大于Tris/TALP-Tris中的活力（p≤0.001）。在实验2中，离心后向精子沉淀中加入冷冻稀释液并冷冻。将先前用Tris和TALP从附睾中回收的冻融精子分配为不进一步稀释（Tris/TALP-O）以及用Tris（Tris/TALP-Tris）和TALP（Tris/TALP-TALP）进一步稀释（1:1，v/v）并评估质量。在两个孵育时间点，用TALP回收的冻融精子（TALP-O/Tris/TALP）的活力与Tris组（Tris-O/Tris/TALP）相当或显著更高（p = 0.003至p>0.05）。在孵育2小时期间，Tris-Tris中冻融精子的活力和存活率显著降低（活力p = 0.007，存活率p = 0.01）。在两个实验中，两种稀释剂类型（Tris与TALP）和孵育时间（0与2小时）均未显著影响低渗条件下的精子膜完整性（p>0.05）。根据对新鲜稀释和冻融精子质量的有益影响表明，精子-TALP可作为从附睾中回收精子以及在家猫中稀释附睾精子的替代培养基。
