Department of Nutritional and Physiological Chemistry, University of Vienna, Althanstrasse 14, 1090, Vienna, Austria.
Am J Chin Med. 2013;41(3):531-44. doi: 10.1142/S0192415X13500389.
This study was designed to compare the anti-inflammatory potential of a Magnolia officinalis L. bark extract solely or in combination with extracts prepared from either Polygonum aviculare L., Sambucus nigra L., or Isodon japonicus L. in bacterial lipopolysaccharide (LPS) stimulated human gingival fibroblasts (HGF-1) and human U-937 monocytes, as cell models of periodontal disease. HGF-1 and U-937 cells were incubated with LPS from either Porphyromonas gingivalis or Escherichia coli together with the four plant extracts alone or in combination. Secretion of anti-inflammatory cytokines from HGF-1 and U-937 cells was measured by means of a multiplexed bead assay system. Magnolia officinalis L. bark extract, at concentrations of 1 μg/mL and 10 μg/mL, reduced interleukin 6 (IL-6) and interleukin-8 (IL-8) secretion from HGF-1 cells to 72.5 ± 28.6% and reduced matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) secretion from U-937 cells to 8.87 ± 7.97% compared to LPS-treated cells (100%). The other three extracts also reduced secretion of these inflammatory markers but were not as effective. Combination of 9 μg/mL Magnolia officinalis L. extract with 1 μg/mL of each of the other extracts maintained the anti-inflammatory effect of Magnolia officinalis L. extract. Combination of 5 μg/mL Magnolia officinalis L. extract with 5 μg/mL Isodon japonicus L. extract also maintained the anti-inflammatory potential of the Magnolia officinalis L. extract, whereas increasing concentrations of any of the other plant extracts in the combination experiments reduced the Magnolia officinalis L. extract efficacy in U-937 cells.
本研究旨在比较厚朴树皮提取物单独或与从蓼属、黑接骨木或黄芩中提取的提取物联合应用于牙龈成纤维细胞（HGF-1）和人 U-937 单核细胞的抗炎潜力，这些细胞是牙周病的细胞模型。用牙龈卟啉单胞菌或大肠杆菌 LPS 刺激 HGF-1 和 U-937 细胞，单独或联合使用四种植物提取物孵育。通过多重珠粒分析系统测量 HGF-1 和 U-937 细胞中抗炎细胞因子的分泌。厚朴树皮提取物在 1μg/mL 和 10μg/mL 浓度下，使 HGF-1 细胞分泌的白细胞介素 6（IL-6）和白细胞介素-8（IL-8）减少至 72.5±28.6%，使 U-937 细胞分泌的基质金属蛋白酶 2（MMP-2）和基质金属蛋白酶 9（MMP-9）减少至 8.87±7.97%，与 LPS 处理的细胞（100%）相比。其他三种提取物也降低了这些炎症标志物的分泌，但效果不如厚朴提取物。9μg/mL 厚朴提取物与每种其它提取物 1μg/mL 的联合使用维持了厚朴提取物的抗炎作用。5μg/mL 厚朴提取物与 5μg/mL 黄芩提取物的联合使用也维持了厚朴提取物的抗炎潜力，而在联合实验中增加任何其它植物提取物的浓度都会降低厚朴提取物在 U-937 细胞中的功效。
