Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Gadjah Mada University, Sekip Utara Yogyakarta-55281, Indonesia.
Asian Pac J Trop Med. 2013 Jun;6(6):438-42. doi: 10.1016/S1995-7645(13)60070-X.
To investigate the hepatoprotective and antioxidant activity of pentagamavunon-0(PGV-0) against CCl4-induced hepatic injury in rats.
The groups of animals were administered with PGV-0 at the doses 2.5, 5, 10, and 20 mg/kg b.w., p.o. once in a day for 6 days and at day 7 the animals were administrated with carbon tetrachloride (CCl) (20%, 2 mL/kg b.w. in liquid paraffin (i.p.). The effect of PGV-0 on serum transaminase (SGPT), alkaline phosphates (ALP) and total bilirubin were determined in CCl4-induced hepatotoxicity in rats. Further, the effects of PGV-0 on glutathione (GSH) content, catalase (CAT) and NO free radical scavenging activity also were investigated.
The results demonstrated that PGV-0 significantly reduced the activity of SGPT, serum ALP and total bilirubin in CCl4 induced rat hepatotoxicity. PGV-0 has effect on the antioxidant and free radical defense system. It prevented the depletion level of GSH and decrease activity of CAT in CCl4-induced liver injury in rats. PGV-0 also demonstrated the free radical scavenger effects on NO free radical scavenging activity with ES value of 32.32 μM.
All of our findings suggests that PGV-0 could protect the liver cells from CCl4-induced liver damages and the mechanism may through the antioxidative effect of PGV-0 to prevent the accumulation of free radicals and protect the liver damage.
研究五甲川嗪-0（PGV-0）对 CCl4 诱导的大鼠肝损伤的保肝和抗氧化活性。
动物分组给予 PGV-0 剂量为 2.5、5、10 和 20mg/kg·bw，po，每天 1 次，连续 6 天，第 7 天给予动物四氯化碳（CCl）（20%，2mL/kg·bw 在液体石蜡中（ip）。测定 PGV-0 对 CCl4 诱导的大鼠肝毒性血清转氨酶（SGPT）、碱性磷酸酶（ALP）和总胆红素的影响。进一步研究了 PGV-0 对谷胱甘肽（GSH）含量、过氧化氢酶（CAT）和 NO 自由基清除活性的影响。
结果表明，PGV-0 能显著降低 CCl4 诱导的大鼠肝毒性中 SGPT、血清 ALP 和总胆红素的活性。PGV-0 对抗氧化和自由基防御系统有影响。它防止了 GSH 的耗竭水平和 CAT 活性的降低在 CCl4 诱导的肝损伤大鼠。PGV-0 还对 NO 自由基清除活性具有自由基清除作用，ES 值为 32.32 μM。
我们的所有发现表明，PGV-0 可以保护肝细胞免受 CCl4 诱导的肝损伤，其机制可能是通过 PGV-0 的抗氧化作用防止自由基的积累和保护肝损伤。
