Myocardial Function, NHLI, Imperial College London, London, W12 0NN, UK,
J Muscle Res Cell Motil. 2013 Aug;34(3-4):189-97. doi: 10.1007/s10974-013-9347-8. Epub 2013 May 28.
We determined the isoforms of tropomyosin expressed and the level of tropomyosin phosphorylation in donor, end-stage failing and hypertrophic obstructive cardiomyopathy samples of human heart muscle. Western blots and isoform-specific antibodies showed that α-tropomyosin was the only significant isoform expressed and that tropomyosin was 25-30% phosphorylated at serine 283. Mass spectrometry confirmed directly that α-tropomyosin made up over 95% of tropomyosin but also indicated the presence of up to 4% κ-tropomyosin and much smaller amounts of β-, γ- and smooth β-tropomyosin and about 26% phosphorylation. Neither the isoform distribution nor the level of phosphorylation changed significantly in the pathological heart muscle samples.
我们测定了人心脏肌肉供体、终末期衰竭和肥厚型梗阻性心肌病样本中表达的原肌球蛋白异构体和原肌球蛋白磷酸化水平。Western blot 和异构体特异性抗体表明，α-原肌球蛋白是唯一显著表达的异构体，丝氨酸 283 处的原肌球蛋白被磷酸化 25-30%。质谱分析直接证实α-原肌球蛋白占原肌球蛋白的 95%以上，但也表明存在高达 4%的κ-原肌球蛋白以及更小比例的β-、γ-和平滑肌β-原肌球蛋白，以及约 26%的磷酸化。在病理性心肌样本中，异构体分布和磷酸化水平均无明显变化。
