Laboratory of Bio-Separation and Analytical Biochemistry, State Key Laboratory of Microbial Metabolism and School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
Electrophoresis. 2013 Aug;34(15):2185-92. doi: 10.1002/elps.201300104. Epub 2013 Jul 15.
Here, a simple micro free-flow electrophoresis (μFFE) was developed for fluorescence sensing of monosaccharide via supermolecule interaction of synthesized boronic acid functionalized benzyl viologen (ο-BBV) and fluorescent dye. The μFFE contained two open electrode cavities and an ion-exchange membrane was sandwiched between two polymethylmethacrylate plates. The experiments demonstrated the following merits of developed μFFE: (i) up to 90.5% of voltage efficiency due to high conductivity of ion-exchange membrane; (ii) a strong ability against influence of bubble produced in two electrodes due to open design of electrode cavities; and (iii) reusable and washable separation chamber (45 mm × 17 mm × 100 μm, 77 μL) avoiding the discard of μFFE due to blockage of solute precipitation in chamber. Remarkably, the μFFE was first designed for the sensing of monosaccharide via the supermolecule interaction of synthesized ο-BBV, fluorescent dye, and monosaccharide. Under the optimized conditions, the minimum concentration of monosaccharide that could be detected was 1 × 10(-11) M. Finally, the developed device was used for the detection of 0.3 mM glucose spiked in human urine. All of the results demonstrated the feasibility of monosaccharide detection via the μFFE.
这里，开发了一种简单的微自由流电泳（μFFE），通过合成的硼酸功能化苄基紫精（ο-BBV）和荧光染料的超分子相互作用，用于单糖的荧光传感。μFFE 包含两个开放的电极腔，离子交换膜夹在两个聚甲基丙烯酸甲酯板之间。实验证明了所开发的 μFFE 的以下优点：（i）由于离子交换膜的高导电性，最高可达 90.5%的电压效率；（ii）由于电极腔的开放式设计，对电极中产生的气泡的影响具有很强的抵抗力；以及（iii）可重复使用和可清洗的分离室（45mm×17mm×100μm，77μL），避免了由于室内容物沉淀堵塞而丢弃 μFFE。值得注意的是，μFFE 首次被设计用于通过合成的 ο-BBV、荧光染料和单糖的超分子相互作用来检测单糖。在优化条件下，可检测到的单糖最低浓度为 1×10(-11) M。最后，该设备用于检测人尿中添加的 0.3mM 葡萄糖。所有结果都证明了通过 μFFE 进行单糖检测的可行性。
