Department of Anatomy, Physiology and Genetics, Uniformed Services University, Bethesda, MD 20814, USA.
Electrophoresis. 2013 Aug;34(15):2229-33. doi: 10.1002/elps.201300077. Epub 2013 Jul 8.
Mild traumatic brain injury (mTBI), especially when it is repeated (rmTBI), can lead to progressive degenerative diseases and lasting neuropsychiatric abnormalities. To better understand the long-term pathobiological changes in mTBI and rmTBI, we exposed rats to single or repeated (5 total; administered on consecutive days) mild blast overpressure, monitored changes in physiological parameters, and determined the plasma levels of select biomarkers at 42 days post injury by proteomics. We unexpectedly found comparable changes in arterial oxygen saturation levels and heart rates of single-injured (SI) and multiple-injured (MI) rats throughout the observation period. Our analyses indicated lasting oxidative stress, vascular abnormalities, and neuronal and glial cell loss in both injured groups. However, MI rats exhibited a relatively more pronounced increase in the plasma levels of most of the tested markers-particularly those associated with inflammation-albeit the differences between the two injured groups were not statistically significant. Our findings indicate that the frequency of blast exposures is an important determinant of the resulting cumulative damage in rmTBI.
轻度创伤性脑损伤（mTBI），尤其是当它反复发生（rmTBI）时，可导致进行性退行性疾病和持久的神经精神异常。为了更好地了解 mTBI 和 rmTBI 的长期病理生物学变化，我们使大鼠暴露于单次或重复（共 5 次；连续天给药）轻度爆震过压下，监测生理参数的变化，并通过蛋白质组学在损伤后 42 天测定选定生物标志物的血浆水平。我们出人意料地发现，在整个观察期间，单次损伤（SI）和多次损伤（MI）大鼠的动脉血氧饱和度水平和心率都有可比的变化。我们的分析表明，在两个损伤组中都存在持久的氧化应激、血管异常以及神经元和神经胶质细胞丢失。然而，尽管两个损伤组之间的差异没有统计学意义，但 MI 大鼠表现出大多数测试标志物的血浆水平相对更明显的升高，特别是那些与炎症相关的标志物。我们的研究结果表明，爆震暴露的频率是 rmTBI 中累积损伤的重要决定因素。
