Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, PR China.
Electrophoresis. 2013 Aug;34(15):2162-8. doi: 10.1002/elps.201300138.
In this study, a fast, low-cost, and facile spray method was proposed. This method deposits highly sensitive surface-enhanced Raman scattering (SERS) silver nanoparticles (AgNPs) on the paper-microfluidic scheme. The procedures for substrate preparation were studied including different strategies to synthesize AgNPs and the optimization of spray cycles. In addition, the morphologies of the different kinds of paper substrates were characterized by SEM and investigated by their SERS signals. The established method was found to be favorable for obtaining good sensitivity and reproducible results. The RSDs of Raman intensity of randomly analyzing 20 spots on the same paper or different filter papers depositing AgNPs are both below 15%. The SERS enhancement factor is approximately 2 × 10(7) . The whole fabrication is very rapid, robust, and does not require specific instruments. Furthermore, the total cost for 1000 pieces of chip is less than $20. These advantages demonstrated the potential for growing SERS applications in the area of environmental monitoring, food safety, and bioanalysis in the future.
在这项研究中，提出了一种快速、低成本且简便的喷雾方法。该方法在纸质微流控方案上沉积了高灵敏度的表面增强拉曼散射（SERS）银纳米粒子（AgNPs）。研究了包括不同策略合成 AgNPs 和优化喷雾循环在内的基底制备程序。此外，通过 SEM 对不同种类的纸质基底的形貌进行了表征，并通过 SERS 信号进行了研究。结果表明，该方法有利于获得良好的灵敏度和可重复性结果。在同一张纸或不同滤纸沉积 AgNPs 的 20 个随机分析点的拉曼强度的 RSD 均低于 15%。SERS 增强因子约为 2×10(7)。整个制造过程非常快速、稳健，且不需要特定的仪器。此外，1000 个芯片的总费用低于 20 美元。这些优势表明，SERS 在未来的环境监测、食品安全和生物分析等领域的应用具有很大的发展潜力。
