Liu Li, Wang Shu-fei, Xu Rui, Peng Xiao-ming
Department of Traditional Chinese and Western Medicine, Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2013 Mar;33(3):380-4.
To observe the effects of Guipi Pill (GPP) on bone marrow cell cycle of mice exposed to benzene and to explore its possible mechanisms for regulating hematopoiesis.
Seventy-two Kunming mice were randomly divided into 6 groups, i.e., the normal control group, the model group, the Western medicine treatment group, the large, middle, and small dose GPP groups, 12 in each group. The mice were exposed to manually simulated high concentrations of benzene fqr eight h every day, fourteen successive days, to replicate benzene intoxication model. Mice in the large, middle, and small dose GPP groups were administered with 8, 4, 2 mg/kg GPP per day respectively by gastrogavage. Mice in the Western medicine treatment group were administered with leucogen (at the daily dose of 1.5 mg/kg) and batyl alcohol (at the daily dose of 5 mg/kg) by gastrogavage. Mice in the model group and the normal control group were administered with normal saline by gastrogavage, once daily, for 3 successive weeks. The nucleated bone marrow cell count and the cell cycle of bone marrow cells were detected using flow cytometry.
Compared with the normal control group, the nucleated bone marrow cell count obviously decreased in the model group (P <0.05). Compared with the model group, the nucleated bone marrow cell count obviously increased in the large and small dose GPP groups, and the Western medicine treatment group (P <0.01). Compared with the normal control group, the S phase cell ratio and proliferation index (PI) increased, and the G0/G1 phase cell ratio decreased in the model group, showing statistical difference (P <0.05). The G0/G1 phase cell ratio decreased, while the G2/M phase cell ratio and PI increased in the large dose GPP group. The S phase cell ratio decreased in the middle dose GPP group, showing statistical difference when compared with the model group (P <0.01, P <0.05). Compared with the Western medicine treatment group, the G2/M phase cell ratio and PI increased, and the G0/G1 phase cell ratio decreased in the large dose GPP group, showing statistical difference (P <0.01).
GPP could promote the recovery of hematopoietic functions of benzene exposed mice by ending off G1 or G2-phase arrest, accelerating G0/G1-S phase and S-G2/M phase transition, promoting the proliferation of bone marrow hematopoietic cells, and improving the peripheral hemogram.
观察归脾丸（GPP）对苯染毒小鼠骨髓细胞周期的影响，探讨其调控造血的可能机制。
将72只昆明小鼠随机分为6组，即正常对照组、模型组、西药治疗组、GPP大、中、小剂量组，每组12只。每天人工模拟高浓度苯染毒小鼠8小时，连续14天，复制苯中毒模型。GPP大、中、小剂量组小鼠分别按每天8、4、2mg/kg灌胃给予GPP。西药治疗组小鼠按每天1.5mg/kg鲨肝醇和5mg/kg肌苷灌胃给药。模型组和正常对照组小鼠按每天灌胃给予生理盐水，连续3周。采用流式细胞术检测有核骨髓细胞计数及骨髓细胞周期。
与正常对照组比较，模型组有核骨髓细胞计数明显减少（P<0.05）。与模型组比较，GPP大、小剂量组及西药治疗组有核骨髓细胞计数明显增加（P<0.01）。与正常对照组比较，模型组S期细胞比例及增殖指数（PI）升高，G0/G1期细胞比例降低，差异有统计学意义（P<0.05）。GPP大剂量组G0/G1期细胞比例降低，G2/M期细胞比例及PI升高。GPP中剂量组S期细胞比例降低，与模型组比较差异有统计学意义（P<0.01，P<0.05）。与西药治疗组比较，GPP大剂量组G2/M期细胞比例及PI升高，G0/G1期细胞比例降低，差异有统计学意义（P<0.01）。
归脾丸可通过解除G1期或G2期阻滞，加速G0/G1-S期及S-G2/M期转换，促进骨髓造血细胞增殖，改善外周血象，从而促进苯染毒小鼠造血功能的恢复。
