Wang Yubo, Ma Chi, Xu Ning, Xu Kan, Wang Honglei, Yu Jinlu, Li Yang, Wang Kuizhong, Wang Xu, Luo Qi
Department of Neurosurgery, The First Hospital of Jilin University , Changchun , P. R. China.
Br J Neurosurg. 2013 Dec;27(6):779-82. doi: 10.3109/02688697.2013.799633. Epub 2013 May 29.
To find a safe, effective, and simple method to create a rabbit aneurysm model.
Twelve adult New Zealand rabbits were divided into two groups: experimental (n = 9) and control (n = 3). The origin of the right external carotid artery was occluded with a temporary aneurysm clip, after which the right external carotid artery was ligated distally. The resulting stump was infused with elastase (experimental group) or saline (control group) for 20 minutes, and the clip was removed. The animals were studied angiographically and sacrificed 3 weeks after the procedure to harvest the aneurysms for histological analysis.
All nine rabbits in the experimental group successfully developed aneurysm after 3 weeks. Aneurysms had an average height of 5.2 ± 1.1 mm and width of 3.5 ± 0.8 mm. Histological analysis showed that the tunica media of the aneurysmal wall was barely identifiable. None of the three rabbits in the control group developed an aneurysm, and instead had achieved closure of the right external carotid artery stump.
We have established an effective and a simple elastase-based method to create a rabbit aneurysm model. The procedure is easy to perform and does not require an interventional technique and or ligation of the internal carotid artery, which would otherwise reduce the blood supply to the brain.
寻找一种安全、有效且简便的方法来建立兔动脉瘤模型。
将12只成年新西兰兔分为两组：实验组（n = 9）和对照组（n = 3）。用临时动脉瘤夹夹闭右侧颈外动脉起始部，然后在远端结扎右侧颈外动脉。将弹性蛋白酶（实验组）或生理盐水（对照组）注入所得残端20分钟，然后移除夹子。术后3周对动物进行血管造影研究，并处死动物以获取动脉瘤进行组织学分析。
实验组的9只兔在3周后均成功形成动脉瘤。动脉瘤平均高度为5.2±1.1毫米，宽度为3.5±0.8毫米。组织学分析显示，动脉瘤壁的中膜几乎无法辨认。对照组的3只兔均未形成动脉瘤，而是右侧颈外动脉残端实现了闭合。
我们建立了一种有效且简便的基于弹性蛋白酶的兔动脉瘤模型创建方法。该操作易于实施，不需要介入技术和/或结扎颈内动脉，否则会减少脑供血。
