State Key Laboratory of Organic Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
Chemosphere. 2013 Sep;93(1):61-8. doi: 10.1016/j.chemosphere.2013.04.068. Epub 2013 May 25.
The biosorption isotherms for phenanthrene (Phen) by cultured algae, field-collected plankton, and market algae samples (OSs) and their fractions (lipid-LP, lipid free carbon-LF, alkaline nonhydrolyzable carbon-ANHC, and acid nonhydrolyzable carbon-NHC) were established. All the biosorption isotherms are well fitted by the Freundlich model. The biosorption isotherms for the ANHC and NHC fractions are nonlinear and for the other fractions are linear. It was found that the NHC fractions are chemically and structurally different from other fractions by using elemental analysis and Fourier transformed infrared spectroscopy (FTIR), consisting mainly of aliphatic polymethylene carbon. The average KOC values for Phen at Ce=0.005Sw are 10706±2768mLg(-1) and 95843±55817mLg(-1) for the bulk market algal samples and their NHC isolates, respectively. As the NHC fraction for Porphyra contains higher polymethylene carbon than that for Seaweed or Spirulina, it exhibits higher biosorption capacity. Moreover, the logKOC values are significantly higher for the field-collected samples than for the market algae and cultured algae samples. The multivariate correlation shows that the logKOC values are positively related to the LP contents, and negatively to the C/N ratios for the original algal samples. Furthermore, the logKOC values are negatively related to the polarity indices (O/C and O+N/C) for the original samples and their fractions excluding LP fractions. These observations help to understand the role of polarity, LP and NHC fractions, and aliphatic structures in the biosorption of Phen, which requires more attention in the examination of sorption processes in the natural environment.
建立了培养藻类、野外采集浮游生物和市售藻类样品（OS）及其各部分（脂类-LP、无脂碳-LF、碱性非水解碳-ANHC 和酸性非水解碳-NHC）对菲的生物吸附等温线。所有生物吸附等温线均很好地符合 Freundlich 模型。ANHC 和 NHC 部分的生物吸附等温线是非线性的，而其他部分的生物吸附等温线是线性的。通过元素分析和傅里叶变换红外光谱（FTIR）发现，NHC 部分在化学和结构上与其他部分不同，主要由脂肪多亚甲基碳组成。在 Ce=0.005Sw 时，Phen 的平均 KOC 值分别为 10706±2768mLg(-1)和 95843±55817mLg(-1)，对于散装市售藻类样品及其 NHC 分离物。由于紫菜的 NHC 部分比海藻或螺旋藻含有更高的多亚甲基碳，因此表现出更高的生物吸附能力。此外，野外采集的样品的 logKOC 值明显高于市售藻类和培养藻类样品。多元相关分析表明，logKOC 值与原始藻类样品的 LP 含量呈正相关，与 C/N 比值呈负相关。此外，logKOC 值与原始样品及其各部分（不包括 LP 部分）的极性指数（O/C 和 O+N/C）呈负相关。这些观察结果有助于理解极性、LP 和 NHC 部分以及脂肪结构在菲生物吸附中的作用，在自然环境中考察吸附过程时需要更加关注这一点。
