光动力疗法（UVA/核黄素）对金黄色葡萄球菌的抗菌药敏性。
Antimicrobial susceptibility of photodynamic therapy (UVA/riboflavin) against Staphylococcus aureus.
作者信息
Kashiwabuchi Renata Tiemi, Khan Yasin, Carvalho Fabio Ramos de Souza, Hirai Flavio, Campos Mauro Silveira, McDonnell Peter John
机构信息
Department of Ophthalmology, Escola Paulista de Medicina, Universidade Federal de São Paulo - UNIFESP, São Paulo, Brazil.
出版信息
Arq Bras Oftalmol. 2012 Nov-Dec;75(6):423-6. doi: 10.1590/s0004-27492012000600011.
PURPOSE
To assess S. aureus in vitro viability after the exposure to ultraviolet light A (UVA) and riboflavin (B2).
METHODS
Samples of S. aureus in 96 well plates (in triplicate) were exposed to riboflavin (B2) and ultraviolet light A (365 nm wavelength) at a power density of 3 mW/cm², 8 mm spot diameter, for 30 minutes. Control groups were prepared as well in triplicate: blank control, ultraviolet light A only, riboflavin only and dead bacteria Control. The bacterial viability was measured using fluorescent microscopy. In order to investigate the occurrence of "viable but non-culturable" microorganisms after treatment, the cell viability was also investigated by plate culture procedure onto a broth medium. Statistical analysis was performed using the triplicate values from each experimental condition.
RESULTS
No difference was observed among the treatment group and the control samples (p=1).
CONCLUSION
The combination of riboflavin 0.1% and ultraviolet light A at 365 nm did not exhibit antimicrobial activity against oxacillin susceptible S. aureus.
目的
评估金黄色葡萄球菌在暴露于紫外线A（UVA）和核黄素（B2）后的体外生存能力。
方法
将96孔板中的金黄色葡萄球菌样本（一式三份）暴露于核黄素（B2）和波长为365nm、功率密度为3mW/cm²、光斑直径为8mm的紫外线A下30分钟。同时也制备了一式三份的对照组：空白对照、仅紫外线A、仅核黄素和死菌对照。使用荧光显微镜测量细菌的生存能力。为了研究处理后“活的但不可培养的”微生物的出现情况，还通过在肉汤培养基上进行平板培养程序来研究细胞活力。使用每个实验条件下的一式三份值进行统计分析。
结果
在处理组和对照样本之间未观察到差异（p = 1）。
结论
0.1%的核黄素与365nm的紫外线A组合对苯唑西林敏感的金黄色葡萄球菌没有抗菌活性。
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