• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
BMP-2 differentially modulates FGF-2 isoform effects in osteoblasts from newborn transgenic mice.BMP-2 差异调节成骨细胞中 FGF-2 同工型的作用来自新生转基因小鼠。
Endocrinology. 2013 Aug;154(8):2723-33. doi: 10.1210/en.2013-1025. Epub 2013 May 28.
2
Reduced expression and function of bone morphogenetic protein-2 in bones of Fgf2 null mice.成纤维细胞生长因子2基因敲除小鼠骨骼中骨形态发生蛋白-2的表达及功能降低。
J Cell Biochem. 2008 Apr 15;103(6):1975-88. doi: 10.1002/jcb.21589.
3
Membrane and integrative nuclear fibroblastic growth factor receptor (FGFR) regulation of FGF-23.膜及整合素核成纤维细胞生长因子受体（FGFR）对FGF-23的调控
J Biol Chem. 2015 Apr 17;290(16):10447-59. doi: 10.1074/jbc.M114.609230. Epub 2015 Mar 9.
4
FGF-2 enhances Runx-2/Smads nuclear localization in BMP-2 canonical signaling in osteoblasts.成纤维细胞生长因子-2（FGF-2）增强骨形态发生蛋白-2（BMP-2）经典信号通路中 Runx-2/Smads 入核定位。
J Cell Physiol. 2013 Nov;228(11):2149-58. doi: 10.1002/jcp.24382.
5
Nuclear fibroblast growth factor 2 (FGF2) isoforms inhibit bone marrow stromal cell mineralization through FGF23/FGFR/MAPK in vitro.核成纤维细胞生长因子 2（FGF2）异构体通过 FGF23/FGFR/MAPK 在体外抑制骨髓基质细胞矿化。
J Bone Miner Res. 2013 Jan;28(1):35-45. doi: 10.1002/jbmr.1721.
6
Signaling pathways implicated in PGF2alpha effects on Fgf2+/+ and Fgf2-/- osteoblasts.涉及 PGF2alpha 对 Fgf2+/+和 Fgf2-/-成骨细胞影响的信号通路。
J Cell Physiol. 2010 Aug;224(2):465-74. doi: 10.1002/jcp.22143.
7
Effect of overexpressing fibroblast growth factor 2 protein isoforms in osteoblastic ROS 17/2.8 cells.在成骨细胞ROS 17/2.8细胞中过表达成纤维细胞生长因子2蛋白亚型的作用。
J Cell Biochem. 2003 Aug 15;89(6):1291-301. doi: 10.1002/jcb.10589.
8
Expression of the high molecular weight fibroblast growth factor-2 isoform of 210 amino acids is associated with modulation of protein kinases C delta and epsilon and ERK activation.由210个氨基酸组成的高分子量成纤维细胞生长因子-2亚型的表达与蛋白激酶Cδ和ε的调节以及细胞外信号调节激酶（ERK）的激活相关。
J Biol Chem. 2001 Jan 12;276(2):1545-54. doi: 10.1074/jbc.M001184200.
9
Regulatory effects of fibroblast growth factor-8 and tumor necrosis factor-α on osteoblast marker expression induced by bone morphogenetic protein-2.成纤维细胞生长因子-8和肿瘤坏死因子-α对骨形态发生蛋白-2诱导的成骨细胞标志物表达的调节作用
Peptides. 2015 Nov;73:88-94. doi: 10.1016/j.peptides.2015.09.007. Epub 2015 Sep 26.
10
Relaxin augments BMP-2-induced osteoblast differentiation and bone formation.松弛素增强 BMP-2 诱导的成骨细胞分化和骨形成。
J Bone Miner Res. 2014 Jul;29(7):1586-96. doi: 10.1002/jbmr.2197.

引用本文的文献

1
Oral thin film containing rice husk liquid smoke effects on the expression of key cytokines and growth factors in a periodontitis model.含稻壳烟熏液的口腔薄膜对牙周炎模型中关键细胞因子和生长因子表达的影响
J Oral Biol Craniofac Res. 2025 Nov-Dec;15(6):1266-1273. doi: 10.1016/j.jobcr.2025.08.007. Epub 2025 Aug 13.
2
A Comparative Study Between the Effectiveness of 980 nm Photobiomodulation Delivered by Hand-Piece With Gaussian vs. Flat-Top Profiles on Osteoblasts Maturation.手持高斯光束和平顶光束980纳米光生物调节对成骨细胞成熟效果的比较研究
Front Endocrinol (Lausanne). 2019 Feb 20;10:92. doi: 10.3389/fendo.2019.00092. eCollection 2019.
3
Fibroblast Growth Factor 2 and Its Receptors in Bone Biology and Disease.成纤维细胞生长因子2及其受体在骨生物学与疾病中的作用
J Endocr Soc. 2018 May 28;2(7):657-671. doi: 10.1210/js.2018-00105. eCollection 2018 Jul 1.
4
The Effects of Photobiomodulation of 808 nm Diode Laser Therapy at Higher Fluence on the Osteogenic Differentiation of Bone Marrow Stromal Cells.808纳米二极管激光高能量光生物调节疗法对骨髓基质细胞成骨分化的影响
Front Physiol. 2018 Feb 23;9:123. doi: 10.3389/fphys.2018.00123. eCollection 2018.
5
TGF-β Family Signaling in Mesenchymal Differentiation.TGF-β 家族信号在间充质分化中的作用。
Cold Spring Harb Perspect Biol. 2018 May 1;10(5):a022202. doi: 10.1101/cshperspect.a022202.
6
Fibroblast growth factor signaling in skeletal development and disease.成纤维细胞生长因子信号在骨骼发育与疾病中的作用
Genes Dev. 2015 Jul 15;29(14):1463-86. doi: 10.1101/gad.266551.115.
7
microRNA-375 inhibits osteogenic differentiation by targeting runt-related transcription factor 2.微小RNA-375通过靶向与 runt 相关的转录因子2抑制成骨分化。
Exp Ther Med. 2015 Jul;10(1):207-212. doi: 10.3892/etm.2015.2477. Epub 2015 May 7.
8
Plasmid DNA-coding p62 as a bone effective anti-inflammatory/anabolic agent.编码p62的质粒DNA作为一种有效的骨抗炎/合成代谢剂。
Oncotarget. 2015 Feb 28;6(6):3590-9. doi: 10.18632/oncotarget.2884.
9
A nuclear odyssey: fibroblast growth factor-2 (FGF-2) as a regulator of nuclear homeostasis in the nervous system.一场核之旅：成纤维细胞生长因子-2（FGF-2）作为神经系统核稳态的调节因子
Cell Mol Life Sci. 2015 May;72(9):1651-62. doi: 10.1007/s00018-014-1818-6. Epub 2015 Jan 1.
10
Dimerization capacities of FGF2 purified with or without heparin-affinity chromatography.经肝素亲和层析或未经肝素亲和层析纯化的FGF2的二聚化能力。
PLoS One. 2014 Oct 9;9(10):e110055. doi: 10.1371/journal.pone.0110055. eCollection 2014.

本文引用的文献

1
FGF-2 enhances Runx-2/Smads nuclear localization in BMP-2 canonical signaling in osteoblasts.成纤维细胞生长因子-2（FGF-2）增强骨形态发生蛋白-2（BMP-2）经典信号通路中 Runx-2/Smads 入核定位。
J Cell Physiol. 2013 Nov;228(11):2149-58. doi: 10.1002/jcp.24382.
2
Fibroblast growth factor-2 and bone morphogenetic protein-2 have a synergistic stimulatory effect on bone formation in cell cultures from elderly mouse and human bone.成纤维细胞生长因子-2 和骨形态发生蛋白-2 对老年鼠和人骨细胞培养物中的骨形成具有协同刺激作用。
J Gerontol A Biol Sci Med Sci. 2013 Oct;68(10):1170-80. doi: 10.1093/gerona/glt018. Epub 2013 Mar 26.
3
Signaling pathways implicated in PGF2alpha effects on Fgf2+/+ and Fgf2-/- osteoblasts.涉及 PGF2alpha 对 Fgf2+/+和 Fgf2-/-成骨细胞影响的信号通路。
J Cell Physiol. 2010 Aug;224(2):465-74. doi: 10.1002/jcp.22143.
4
FGF2-activated ERK mitogen-activated protein kinase enhances Runx2 acetylation and stabilization.成纤维细胞生长因子 2 激活的细胞外信号调节激酶丝裂原活化蛋白激酶增强 runt 相关转录因子 2 的乙酰化和稳定性。
J Biol Chem. 2010 Feb 5;285(6):3568-3574. doi: 10.1074/jbc.M109.055053. Epub 2009 Dec 10.
5
Nuclear isoforms of fibroblast growth factor 2 are novel inducers of hypophosphatemia via modulation of FGF23 and KLOTHO.成纤维细胞生长因子 2 的核异构体通过调节成纤维细胞生长因子 23 和 klotho 是低磷血症的新型诱导剂。
J Biol Chem. 2010 Jan 22;285(4):2834-46. doi: 10.1074/jbc.M109.030577. Epub 2009 Nov 20.
6
Mechanotransduction in osteoblast regulation and bone disease.成骨细胞调节与骨疾病中的机械转导
Trends Mol Med. 2009 May;15(5):208-16. doi: 10.1016/j.molmed.2009.03.001. Epub 2009 Apr 8.
7
Akt promotes BMP2-mediated osteoblast differentiation and bone development.Akt促进BMP2介导的成骨细胞分化和骨骼发育。
J Cell Sci. 2009 Mar 1;122(Pt 5):716-26. doi: 10.1242/jcs.042770. Epub 2009 Feb 10.
8
Fibroblast growth factor receptor 2 promotes osteogenic differentiation in mesenchymal cells via ERK1/2 and protein kinase C signaling.成纤维细胞生长因子受体2通过细胞外信号调节激酶1/2和蛋白激酶C信号通路促进间充质细胞的成骨分化。
J Biol Chem. 2009 Feb 20;284(8):4897-904. doi: 10.1074/jbc.M805432200. Epub 2008 Dec 30.
9
Endogenous FGF-2 is critically important in PTH anabolic effects on bone.内源性成纤维细胞生长因子-2在甲状旁腺激素对骨骼的合成代谢作用中至关重要。
J Cell Physiol. 2009 Apr;219(1):143-51. doi: 10.1002/jcp.21661.
10
Exported 18-kDa isoform of fibroblast growth factor-2 is a critical determinant of bone mass in mice.成纤维细胞生长因子2的18-kDa分泌型异构体是小鼠骨量的关键决定因素。
J Biol Chem. 2009 Jan 30;284(5):3170-3182. doi: 10.1074/jbc.M804900200. Epub 2008 Dec 4.

BMP-2 差异调节成骨细胞中 FGF-2 同工型的作用来自新生转基因小鼠。

BMP-2 differentially modulates FGF-2 isoform effects in osteoblasts from newborn transgenic mice.

机构信息

School of Biosciences and Biotechnology, University of Camerino, Camerino, MC, 62032 Italy.

出版信息

Endocrinology. 2013 Aug;154(8):2723-33. doi: 10.1210/en.2013-1025. Epub 2013 May 28.

DOI:10.1210/en.2013-1025
PMID:23715864
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3713219/
Abstract

We previously generated separate lines of transgenic mice that specifically overexpress either the Fibroblast growth factor (FGF)-2 low-molecular-mass isoform (Tg(LMW)) or the high-mass isoforms (Tg(HMW)) in the osteoblast lineage. Vector/control (Tg(Vector)) mice were also made. Here we report the use of isolated calvarial osteoblasts (COBs) from those mice to investigate whether the FGF-2 protein isoforms differentially modulate bone formation in vitro. Our hypothesis states that FGF-2 isoforms specifically modulate bone morphogenetic protein 2 (BMP-2) function and subsequently bone differentiation genes and their related signaling pathways. We found a significant increase in alkaline phosphatase-positive colonies in Tg(LMW) COBs compared with Tg(Vector) controls. BMP-2 treatment significantly increased mineralized colonies in Tg(Vector) and Tg(LMW) COBs. BMP-2 caused a further significant increase in mineralized colonies in Tg(LMW) COBs compared with Tg(Vector) COBs but did not increase alkaline phosphatase-positive colonies in Tg(HMW) COBs. Time-course studies showed that BMP-2 caused a sustained increase in phosphorylated mothers against decapentaplegic-1/5/8 (Smad/1/5/8), runt-related transcription factor-2 (Runx-2), and osterix protein in Tg(LMW) COBs. BMP-2 caused a sustained increase in phospho-p38 MAPK in Tg(Vector) but only a transient increase in Tg(LMW) and Tg(HMW) COBs. BMP-2 caused a transient increase in phospho-p44/42 MAPK in Tg(Vector) COBs and no increase in Tg(LMW) COBs, but a sustained increase was found in Tg(HMW) COBs. Basal expression of FGF receptor 1 protein was significantly increased in Tg(LMW) COBs relative to Tg(Vector) COBs, and although BMP-2 caused a transient increase in FGF receptor 1 expression in Tg(Vector) COBs and Tg(HMW) COBs, there was no further increase Tg(LMW) COBs. Interestingly, although basal expression of FGF receptor 2 was similar in COBs from all genotypes, BMP-2 treatment caused a sustained increase in Tg(LMW) COBs but decreased FGF receptor 2 in Tg(Vector) COBs and Tg(HMW) COBs.

摘要

我们之前生成了分别在成骨细胞系中特异性过表达成纤维细胞生长因子 (FGF)-2 低分子量同种型 (Tg[LMW]) 或高分子量同种型 (Tg[HMW]) 的转基因小鼠系。还制作了载体/对照 (Tg[Vector]) 小鼠。在这里，我们报告使用来自这些小鼠的分离的颅盖骨成骨细胞 (COB) 来研究 FGF-2 蛋白同种型是否在体外差异调节骨形成。我们的假设是，FGF-2 同种型特异性调节骨形态发生蛋白 2 (BMP-2) 的功能，随后调节骨分化基因及其相关信号通路。我们发现 Tg[LMW] COB 中的碱性磷酸酶阳性集落数量明显增加，与 Tg[Vector]对照相比。BMP-2 处理显着增加了 Tg[Vector]和 Tg[LMW] COB 中的矿化集落。BMP-2 导致 Tg[LMW] COB 中的矿化集落进一步显着增加，与 Tg[Vector] COB 相比，但未增加 Tg[HMW] COB 中的碱性磷酸酶阳性集落。时程研究表明，BMP-2 导致 Tg[LMW] COB 中的磷酸化 mothers against decapentaplegic-1/5/8 (Smad/1/5/8)、 runt-related transcription factor-2 (Runx-2) 和 osterix 蛋白持续增加。BMP-2 导致 Tg[Vector]中的磷酸化 p38 MAPK 持续增加，但仅在 Tg[LMW]和 Tg[HMW] COB 中短暂增加。BMP-2 导致 Tg[Vector] COB 中的磷酸化 p44/42 MAPK 短暂增加，而 Tg[LMW] COB 无增加，但 Tg[HMW] COB 中发现持续增加。与 Tg[Vector] COB 相比，Tg[LMW] COB 中 FGF 受体 1 蛋白的基础表达显着增加，尽管 BMP-2 导致 Tg[Vector] COB 和 Tg[HMW] COB 中 FGF 受体 1 表达短暂增加，但 Tg[LMW] COB 没有进一步增加。有趣的是，尽管所有基因型 COB 中的 FGF 受体 2 基础表达相似，但 BMP-2 处理导致 Tg[LMW] COB 中的持续增加，而 Tg[Vector] COB 和 Tg[HMW] COB 中的 FGF 受体 2 减少。