Wakamatsu Rika, Takenaka Shoji, Ohsumi Tatsuya, Terao Yutaka, Ohshima Hayato, Okiji Takashi
Division of Cariology, Operative Dentistry and Endodontics, Department of Oral Health Science, Niigata University Graduate School of Medical and Dental Sciences, 5274, Gakkocho-dori 2-bancho, Chuo-ku, Niigata, 951-8514, Japan.
Clin Oral Investig. 2014;18(2):625-34. doi: 10.1007/s00784-013-1002-7. Epub 2013 May 29.
The aim of this study was to determine whether different antiseptic mouthrinses show different penetration kinetics into Streptococcus mutans biofilms.
The biofilms, grown on glass-based dishes, were exposed to one of four mouthrinses containing chlorhexidine digluconate, essential oil, cetylpyridinium chloride, or isopropylmethylphenol. Then, penetration velocities were determined by monitoring fluorescence loss of calcein AM-stained biofilms with time-lapse confocal laser scanning microscopy. Bactericidal activity was assessed with fluorescent bacterial viable cell (Live/Dead) staining and viable cell counts. Bacterial detachment after the mouthrinse exposure was determined by measuring fluorescence reduction of SYTO9-stained biofilms.
The essential oil-containing mouthrinse showed significantly faster penetration velocity than the other mouthrinses (ANCOVA and Bonferroni test, p < 0.05). However, even 5 min of exposure left the biofilm structure almost intact. After 30 s (consumer rinsing time) of exposure, the essential oil-containing mouthrinse showed the highest log reduction of viable cells (2.7 log CFU) measured by Live/Dead staining, and the mean reduction of total viable cells was 1.41 log CFU measured by viable cell count.
The essential oil-containing mouthrinse showed the best penetration. Within 30 s of exposure, however, no mouthrinses injured all the microorganisms and all mouthrinses left the biofilm structure nearly intact.
The mouthrinses tested showed different levels of biofilm penetration. The essential oil rinse was superior to other rinses by all three of the in vitro measurements performed.
本研究旨在确定不同的抗菌漱口水对变形链球菌生物膜的渗透动力学是否存在差异。
在玻璃培养皿上生长的生物膜分别暴露于四种含葡萄糖酸洗必泰、精油、西吡氯铵或异丙基甲基苯酚的漱口水之一。然后，通过延时共聚焦激光扫描显微镜监测钙黄绿素AM染色生物膜的荧光损失来确定渗透速度。用荧光细菌活细胞（死活）染色和活细胞计数评估杀菌活性。通过测量SYTO9染色生物膜的荧光减少来确定漱口水暴露后的细菌脱落情况。
含精油的漱口水显示出比其他漱口水显著更快的渗透速度（协方差分析和Bonferroni检验，p<0.05）。然而，即使暴露5分钟，生物膜结构几乎仍保持完整。暴露30秒（消费者漱口时间）后，含精油的漱口水通过死活染色测得的活细胞对数减少最高（2.7 log CFU），通过活细胞计数测得的总活细胞平均减少为1.41 log CFU。
含精油的漱口水显示出最佳的渗透性。然而，在暴露30秒内，没有一种漱口水能杀死所有微生物，且所有漱口水都使生物膜结构几乎保持完整。
所测试的漱口水显示出不同程度的生物膜渗透性。通过所进行的三项体外测量，含精油的漱口水均优于其他漱口水。
