University of Leeds, Dental Institute, Clarendon Way, Leeds LS2 9LU, UK.
Arch Oral Biol. 2011 Sep;56(9):829-36. doi: 10.1016/j.archoralbio.2011.02.001. Epub 2011 Mar 4.
Bacterial biofilms in the mouth are prime mediators of the destruction of the dental and oral tissues. This brief review summarises recent work using a device for generating intact plaque in the mouth on natural enamel surfaces such that quantitative studies of mass transfer through natural plaque biofilms could be carried out in relation to plaque architecture. This data is discussed against the background of existing information. The device revealed complex plaque architecture with high a surface area to mass ratio decreasing from the exterior of the biofilm towards the tissue surface. Fluoride, a potent inhibitor of caries was concentrated in the outer regions of the biofilm. This implies some restriction of diffusion and possibly binding to the high surface area of the outer biofilm. Whilst all components examined conformed to this distribution pattern, some relatively uncharged materials penetrated the bacterial biomass whilst other, more highly charged materials tended to be restricted to the channels or biomass surface. Plaque architecture was robust but could be altered using detergent indicating that biomass architecture and chemistry could be manipulated as a possible means of facilitating mass transport of therapeutics.
口腔中的细菌生物膜是破坏牙齿和口腔组织的主要因素。本综述简要总结了最近使用一种设备在天然牙釉质表面生成完整牙菌斑的工作，从而可以对通过天然牙菌斑生物膜的质量传递进行定量研究，以了解牙菌斑的结构。这些数据是在现有信息的背景下讨论的。该设备揭示了复杂的牙菌斑结构，其表面积与质量比从生物膜的外部向组织表面逐渐降低。氟化物是一种有效的龋齿抑制剂，集中在生物膜的外部区域。这意味着扩散受到一定限制，可能与生物膜外部的高表面积结合。虽然所有检查的成分都符合这种分布模式，但一些相对不带电荷的物质穿透了细菌生物量，而其他带更多电荷的物质则倾向于被限制在通道或生物量表面。牙菌斑结构很坚固，但可以用清洁剂改变，这表明生物量结构和化学成分可以被操纵，以促进治疗药物的质量传递。
