Shah M, Agrawal Y K, Garala K, Ramkishan A
Gujarat Forensic Sciences University, Institute of Research and Development, Near Police Bhavan, Sector 18A, Gandhinagar-382 007, India.
Indian J Pharm Sci. 2012 Sep;74(5):434-42. doi: 10.4103/0250-474X.108419.
The aim of this study was to understand and investigate the relationship between experimental factors and their responses in the preparation of ciprofloxacin hydrochloride based solid lipid nanoparticles. A quadratic relationship was studied by developing central composite rotatable design. Amount of lipid and drug, stirring speed and stirring time were selected as experimental factors while particle size, zeta potential and drug entrapment were used as responses. Prior to the experimental design, a qualitative prescreening study was performed to check the effect of various solid lipids and their combinations. Results showed that changing the amount of lipid, stirring speed and stirring time had a noticeable influence on the entrapment efficiencies and particle size of the prepared solid lipid nanoparticles. The particle size of a solid lipid nanoparticle was in the range of 159-246 nm and drug encapsulation efficiencies were marginally improved by choosing a binary mixture of physically incompatible solid lipids. Release of ciprofloxacin hydrochloride from solid lipid nanoparticle was considerably slow, and it shows Higuchi matrix model as the best fitted model. Study of solid lipid nanoparticle suggested that the lipid based carrier system could potentially be exploited as a delivery system with improved drug entrapment efficiency and controlled drug release for water soluble actives.
本研究的目的是了解和研究在制备盐酸环丙沙星固体脂质纳米粒过程中实验因素与其响应之间的关系。通过开展中心复合旋转设计研究了二次关系。脂质和药物的用量、搅拌速度和搅拌时间被选为实验因素，而粒径、ζ电位和药物包封率则用作响应指标。在进行实验设计之前，开展了一项定性预筛选研究，以检验各种固体脂质及其组合的效果。结果表明，改变脂质用量、搅拌速度和搅拌时间对所制备的固体脂质纳米粒的包封效率和粒径有显著影响。固体脂质纳米粒的粒径在159 - 246 nm范围内，通过选择物理不相容的固体脂质二元混合物，药物包封效率略有提高。盐酸环丙沙星从固体脂质纳米粒中的释放相当缓慢，并且显示Higuchi基质模型为最佳拟合模型。对固体脂质纳米粒的研究表明，基于脂质的载体系统有可能被开发为一种具有更高药物包封效率和可控药物释放的水溶性活性成分递送系统。
