Department of Pediatrics, College of Medicine, Chungbuk National University, Cheongju 361-763, Korea.
J Ginseng Res. 2013 Mar;37(1):94-9. doi: 10.5142/jgr.2013.37.94.
Proteinuric conditions demonstrate structural and compositional changes of the foot processes and slit diaphragms between podocytes. p130Cas in podocytes serves as an adapter protein anchoring glomerular basement membrane to actin filaments of podocyte cytoskeleton. To investigate the effect of ginseng total saponin (GTS) on the pathologic changes of podocyte p130Cas induced by diabetic conditions, we cultured mouse podocytes under: 1) normal glucose (5 mM, control); 2) high glucose (HG, 30 mM); 3) advanced glycosylation endproducts (AGE)-added; or 4) HG plus AGE-added conditions and treated with GTS. In confocal imaging, p130Cas colocalized with zonula occludens-1 and synaptopodin connecting to F-actin. However, diabetic conditions relocalized p130Cas molecules at perinuclear cytoplasmic area and reduced the intensity of p130Cas. In Western blotting, diabetic conditions, especially HG plus AGE-added condition, decreased cellular p130Cas protein levels at 24 and 48 h. GTS improved such quantitative and qualitative changes. These findings imply that HG and AGE have an influence on the redistribution and amount of p130Cas of podocytes, which can be reversed by GTS.
蛋白尿状态表明足细胞的足突和裂孔隔膜的结构和组成发生了变化。足细胞中的 p130Cas 作为衔接蛋白，将肾小球基底膜锚定到足细胞细胞骨架的肌动蛋白丝上。为了研究人参总皂苷 (GTS) 对糖尿病条件下足细胞 p130Cas 病理变化的影响，我们在以下条件下培养小鼠足细胞：1）正常葡萄糖（5mM，对照）；2）高葡萄糖（HG，30mM）；3）晚期糖基化终产物（AGE）添加；或 4）HG 加 AGE 添加条件，并给予 GTS 处理。在共聚焦成像中，p130Cas 与封闭蛋白-1 和突触蛋白共定位，连接到 F-肌动蛋白。然而，糖尿病条件将 p130Cas 分子重新定位到核周细胞质区域，并降低了 p130Cas 的强度。在 Western blot 中，糖尿病条件，特别是 HG 加 AGE 添加条件，在 24 和 48 小时降低了细胞 p130Cas 蛋白水平。GTS 改善了这种定量和定性的变化。这些发现表明，HG 和 AGE 对足细胞 p130Cas 的重分布和数量有影响，GTS 可以逆转这种影响。
