Department of Infectious Diseases, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China.
PLoS One. 2013 May 23;8(5):e64235. doi: 10.1371/journal.pone.0064235. Print 2013.
The metastasis-associated in colon cancer 1 gene (MACC1) has been found to be associated with cancer development and progression. The aim of this study was to investigate the prognostic value of MACC1 in early-stage and AFP-normal hepatocellular carcinoma (HCC).
mRNA and protein levels of MACC1 expression in one normal liver epithelial cells THLE3 and 15 HCC cell lines were examined using reverse transcription-PCR and Western blot. MACC1 expression was also comparatively studied in 6 paired HCC lesions and the adjacent non-cancerous tissue samples. Immunohistochemistry was employed to analyze MACC1 expression in 308 clinicopathologically characterized HCC cases. Statistical analyses were applied to derive association between MACC1 expression scores and clinical staging as well as patient survival.
Levels of MACC1 mRNA and protein were higher in HCC cell lines and HCC lesions than in normal liver epithelial cells and the paired adjacent noncancerous tissues. Significant difference in MACC1 expression was found in patients of different TNM stages (P<0.001). Overall survival analysis showed that high MACC1 expression level correlated with lower survival rate (P = 0.001). Importantly, an inverse correlation between MACC1 level and patient survival remained significant in subjects with early-stage HCC or with normal serum AFP level.
MACC1 protein may represent a promising biomarker for predicting the prognosis of HCC, including in early-stage and AFP-normal patients.
结肠癌转移相关基因 1（MACC1）已被发现与癌症的发展和进展有关。本研究旨在探讨 MACC1 在早期和 AFP 正常肝细胞癌（HCC）中的预后价值。
采用逆转录-PCR 和 Western blot 检测 MACC1 在一个正常肝上皮细胞 THLE3 和 15 个 HCC 细胞系中的 mRNA 和蛋白水平。还比较了 6 对 HCC 病变和相邻非癌组织样本中的 MACC1 表达。免疫组织化学分析了 308 例临床病理特征明确的 HCC 病例中的 MACC1 表达。统计分析用于推导 MACC1 表达评分与临床分期以及患者生存之间的关联。
MACC1 mRNA 和蛋白水平在 HCC 细胞系和 HCC 病变中高于正常肝上皮细胞和配对的相邻非癌组织。MACC1 表达在不同 TNM 分期的患者中存在显著差异（P<0.001）。总生存分析显示，高 MACC1 表达水平与生存率降低相关（P = 0.001）。重要的是，在早期 HCC 或 AFP 正常的患者中，MACC1 水平与患者生存之间的负相关仍然显著。
MACC1 蛋白可能是预测 HCC 预后的有前途的生物标志物，包括早期和 AFP 正常的患者。
