Vanitha Subburaj, Thiagarajan Venkata Rathina Kumar, Muthuraman Arunachalam, Krishnan Shanmugarajan, Aruna Ajithadas, Tharabai R
Department of Pharmacognosy, College of Pharmacy, Madurai Medical College, Madurai, Tamil Nadu, India.
Pharmacology Division, Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, India
Toxicol Ind Health. 2015 Dec;31(12):1185-94. doi: 10.1177/0748233713491808. Epub 2013 May 29.
The present study was designed to investigate the antinociceptive effects of methanolic leaf extract of Swietenia mahagoni (MESM) on acrylamide-induced painful neuropathy in rats. The intraperitoneal administration of acrylamide (30 mg/kg; for 24 consecutive days) has been employed for the induction of painful neuropathy. Acrylamide induced nociceptive pain sensitive changes, which have been assessed by hot plate, Von Frey Hair, and tail immersion tests at different time intervals, that is, 0, 6, 12, 18, and 24th day. Furthermore, the biochemical changes, that is, thiobarbituric acid-reactive substances, reduced glutathione, and total calcium levels have been estimated in sciatic nerve tissue on 24th day and histopathological changes have been observed in sciatic nerve tissue sample. MESM and pregabalin have been administered for 14 consecutive days before 1 h of the each acrylamide injection. Administration of acrylamide resulted in significant changes in behavioral and biochemical parameters. Pretreatment of MESM ameliorated acrylamide-induced behavioral, biochemical, and histopathological changes in a dose-dependent manner, which is similar to that of pregabalin-pretreated group. These findings suggested that the neuroprotective effect of S. mahagoni may be due to its potential of antioxidative, calcium channel modulatory, and neuroprotective action.
本研究旨在探讨桃花心木甲醇叶提取物（MESM）对丙烯酰胺诱导的大鼠疼痛性神经病变的抗伤害感受作用。腹腔注射丙烯酰胺（30 mg/kg；连续24天）用于诱导疼痛性神经病变。丙烯酰胺诱导伤害感受性疼痛敏感变化，在不同时间间隔，即第0、6、12、18和24天，通过热板法、von Frey毛发法和尾浸法进行评估。此外，在第24天对坐骨神经组织中的生化变化，即硫代巴比妥酸反应性物质、还原型谷胱甘肽和总钙水平进行了评估，并观察了坐骨神经组织样本的组织病理学变化。在每次注射丙烯酰胺前1小时，连续14天给予MESM和普瑞巴林。丙烯酰胺的给药导致行为和生化参数发生显著变化。MESM预处理以剂量依赖的方式改善了丙烯酰胺诱导的行为、生化和组织病理学变化，这与普瑞巴林预处理组相似。这些发现表明，桃花心木的神经保护作用可能归因于其抗氧化、钙通道调节和神经保护作用的潜力。
