Hanson Erin K, Ballantyne Jack
National Center for Forensic Science, Orlando, FL, USA.
Methods Mol Biol. 2013;1024:221-34. doi: 10.1007/978-1-62703-453-1_18.
The development of molecular genetics-based body fluid identification methods in forensic science has become necessary to provide greater sensitivity and specificity than that obtained using conventional serological and immunological methods. Numerous studies have demonstrated the ability to identify the body fluid origin of forensically relevant biological stains using messenger RNA expression analysis. However, the length of the amplified products used in these assays may not be ideal for use with highly degraded or environmentally compromised forensic casework samples. Therefore a novel approach to body fluid identification using small RNA profiling (e.g., microRNA or miRNA profiling) was developed in an attempt to improve the success of analysis with highly degraded samples. We have identified a set of nine differentially expressed miRNAs that permit the identification of the body fluid origin of forensic biological stains and in this chapter provide the detailed procedures for performing these assays.
在法医学中，基于分子遗传学的体液鉴定方法的发展已成为必要，以便提供比传统血清学和免疫学方法更高的灵敏度和特异性。大量研究表明，使用信使核糖核酸表达分析能够鉴定法医学相关生物污渍的体液来源。然而，这些检测中使用的扩增产物长度对于高度降解或受环境影响的法医案件样本而言可能并不理想。因此，开发了一种使用小RNA谱分析（例如，微小RNA或miRNA谱分析）进行体液鉴定的新方法，试图提高对高度降解样本的分析成功率。我们已经鉴定出一组九个差异表达的miRNA，它们能够鉴定法医生物污渍的体液来源，并且在本章中提供了进行这些检测的详细程序。
