Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki, Japan.
Acta Histochem Cytochem. 2013 Apr 30;46(2):75-84. doi: 10.1267/ahc.13005. Epub 2013 Apr 12.
Leptin is a hormone mainly produced by white adipose cells, and regulates body fat and food intake by acting on hypothalamus. Leptin receptor is expressed not only in the hypothalamus but in a variety of peripheral tissues, suggesting that leptin has pleiotropic functions. In this study, we investigated the effect of leptin on the progression of peritoneal fibrosis induced by intraperitoneal injection of chlorhexidine gluconate (CG) every other day for 2 or 3 weeks in mice. This study was conducted in male C57BL/6 mice and leptin-deficient ob/ob mice. Peritoneal fluid, blood, and peritoneal tissues were collected 15 or 22 days after CG injection. CG injection increased the level of leptin in serum and peritoneal fluid with thickening of submesothelial compact zone in wild type mice, but CG-injected ob/ob mice attenuate peritoneal fibrosis, and markedly reduced the number of myofibroblasts, infiltrating macrophages, and blood vessels in the thickened submesothelial area. The 2-week leptin administration induced a more thickened peritoneum in the CG-injected C57BL/6 mice than in the PBS group. Our results indicate that an upregulation of leptin appears to play a role in fibrosis and inflammation during peritoneal injury, and reducing leptin may be a therapeutically potential for peritoneal fibrosis.
瘦素主要由白色脂肪细胞产生，通过作用于下丘脑来调节体脂肪和食物摄入。瘦素受体不仅在下丘脑表达，而且在各种外周组织中表达，这表明瘦素有多种功能。在这项研究中，我们研究了瘦素对腹腔注射葡萄糖酸洗必泰（CG）每隔一天诱导的腹膜纤维化进展的影响，在小鼠中进行了 2 或 3 周的实验。这项研究在雄性 C57BL/6 小鼠和瘦素缺乏的 ob/ob 小鼠中进行。CG 注射后 15 或 22 天收集腹膜液、血液和腹膜组织。CG 注射增加了血清和腹膜液中瘦素的水平，导致野生型小鼠的亚上皮致密区增厚，但 CG 注射的 ob/ob 小鼠减轻了腹膜纤维化，并显著减少了增厚的亚上皮区中的肌成纤维细胞、浸润的巨噬细胞和血管的数量。与 PBS 组相比，CG 注射的 C57BL/6 小鼠中 2 周的瘦素给药导致腹膜更厚。我们的结果表明，瘦素的上调似乎在腹膜损伤期间的纤维化和炎症中起作用，减少瘦素可能是腹膜纤维化的一种治疗潜力。
