Sun Li, Fan Xiaoli
Department of Histology and Embryology, College of Basic Medicine of Guilin Medicial University, Guilin, Guangxi 541004, China.
J Biomed Res. 2013 May;27(3):202-7. doi: 10.7555/JBR.27.20120019. Epub 2013 Mar 24.
Lung cancer is one of the most important causes of cancer-related mortality worldwide. Human cytochrome P450 2A13 enzyme (CYP2A13) is predominantly expressed in the respiratory tract and could catalyze various carcinogens. In this study, we quantified CYP2A13 expression in non-small cell lung cancer (NSCLC) tissues and examined the relation between CYP2A13 and clinicopathologic factors. Thirty-five paired lung cancer and normal tissues were studied for the expression of the CYP2A13 gene by using real-time PCR and Western blotting assays. We also investigated the relationship between CYP2A13 expression and clinicopathologic factors such as age, gender, histology and lymph node status in tumor tissues. SPSS (17.0) statistical software was applied for data analysis. The real-time PCR results showed that there was no significant difference in the CYP2A13 mRNA transcript levels between tumor and paired normal tissues in the 35 samples and in 12 paired squamous cell carcinomas. In adenocarcinoma, the expression of CYP2A13 mRNA in tumor tissues was 12.5% of that in adjacent tissues (P < 0.05) and it was not associated with age, gender, histology and lymph node status of the patients. The amounts of CYP2A13 proteins detected by Western blotting assays correlated well with those of the corresponding mRNAs. In conclusion, the expression of CYP2A13 was downregulated in lung adenocarcinoma. CYP2A13 may be involved in the development and progression of lung adenocarcinoma.
肺癌是全球癌症相关死亡的最重要原因之一。人类细胞色素P450 2A13酶（CYP2A13）主要在呼吸道表达，可催化多种致癌物。在本研究中，我们对非小细胞肺癌（NSCLC）组织中的CYP2A13表达进行了定量，并研究了CYP2A13与临床病理因素之间的关系。通过实时PCR和蛋白质印迹分析，对35对肺癌组织和正常组织进行了CYP2A13基因表达研究。我们还研究了肿瘤组织中CYP2A13表达与年龄、性别、组织学和淋巴结状态等临床病理因素之间的关系。应用SPSS（17.0）统计软件进行数据分析。实时PCR结果显示，在35个样本和12对鳞状细胞癌中，肿瘤组织和配对正常组织之间的CYP2A13 mRNA转录水平无显著差异。在腺癌中，肿瘤组织中CYP2A13 mRNA的表达是相邻组织的12.5%（P < 0.05），且与患者的年龄、性别、组织学和淋巴结状态无关。蛋白质印迹分析检测到的CYP2A13蛋白量与相应mRNA的量相关性良好。总之，CYP2A13在肺腺癌中表达下调。CYP2A13可能参与肺腺癌的发生和发展。
