Fulkerson J P, Berke A, Parthasarathy N
Department of Orthopaedic Surgery, University of Connecticut School of Medicine, Farmington.
Am J Sports Med. 1990 May-Jun;18(3):249-53. doi: 10.1177/036354659001800305.
Autogenous patellar tendon grafts were transplanted into the knees of 40 New Zealand White adult rabbits. Grafts were subsequently analyzed for rate of collagen synthesis, collagen content, collagen type, histologic change, and cyanogen bromide cleavage patterns of collagen to closely assess the nature of collagen in tendon grafts up to 2 years from the time of transplantation. Tendon grafts were placed in rabbit knees as free fragments or were attached to synovium. These studies show that tendon grafts, even without vascularization or stress, remain viable after intraarticular transfer. Vascularization produces a trend toward increased collagen synthesis, but statistical analysis suggests that control levels of collagen synthesis continue after tendon transfers into rabbit knees. Cyanogen bromide cleavage peptides showed appropriate collagen formed by unstressed autogenous tendon transplants removed from rabbit knees up to 2 years from transplantation. All tendon grafts degenerated initially, but began to form histologically healthy looking connective tissue by 18 to 24 weeks after transplantation. Overall, the results are encouraging with regard to the fate of intraarticular tendon grafts.
将自体髌腱移植物移植到40只成年新西兰白兔的膝关节中。随后对移植物进行分析，检测其胶原蛋白合成速率、胶原蛋白含量、胶原类型、组织学变化以及胶原蛋白的溴化氰裂解模式，以密切评估移植后长达2年的肌腱移植物中胶原蛋白的性质。肌腱移植物以游离碎片的形式置于兔膝关节中，或与滑膜相连。这些研究表明，即使没有血管化或应力作用，肌腱移植物在关节内移植后仍能存活。血管化会使胶原蛋白合成有增加的趋势，但统计分析表明，肌腱移植到兔膝关节后，胶原蛋白合成水平仍维持在对照水平。溴化氰裂解肽显示，从移植后长达2年的兔膝关节中取出的无应力自体肌腱移植物能形成合适的胶原蛋白。所有肌腱移植物最初都会退化，但在移植后18至24周开始形成组织学上看起来健康的结缔组织。总体而言，这些结果对于关节内肌腱移植物的转归是令人鼓舞的。
