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[从受细菌性火疫病影响的果树上分离出的噬菌体的多价性]

[Polyvalence of bacteriophages isolated from fruit trees, affected by bacterial fire blight].

作者信息

Tovkach F I, Moroz S N, Korol' N A, Faĭdiuk Iu V, Kushkina A I

出版信息

Mikrobiol Z. 2013 Mar-Apr;75(2):80-8.

Abstract

Phage populations appearing as a result of a pathogenic process caused by Erwinia amylovora have been discovered and described. They accompany bacterial fire blight development in the process of quince, pear and apple trees vegetation in Zakarpattya region of Ukraine. Phage isolates of the affected pear and quince include polyvalent virulent phages able to develop on bacterial strains associated with plants--E. amylovora. E. "horticola" and Pantoea agglomerans. E. amylovora isolated from the plant tissues affected by the fire blight and detected at the same time as phages proved to be resistant to the viral infection. It is hard to explain now this characteristic however it was noticed that resistance to phages can change drastically in case of dissociation, lysogenization and mutagenesis of erwinia in laboratory conditions. Phage population study shows that they are heterogeneous and can obviously include not only polyvalent but also specific viruses. Further studies of biology and molecular genetics of pure lines of isolated phages will help to get closer to understanding the place and role of bacteriophages in the complicated network of relations between bacterial pathogens and plants.

摘要

已经发现并描述了由解淀粉欧文氏菌引起的致病过程所产生的噬菌体群体。它们伴随着乌克兰外喀尔巴阡地区榅桲、梨树和苹果树生长过程中细菌性火疫病的发展。受感染的梨和榅桲的噬菌体分离物包括能够在与植物相关的细菌菌株——解淀粉欧文氏菌、“园艺欧文氏菌”和成团泛菌上生长的多价烈性噬菌体。从受火疫病影响的植物组织中分离出的解淀粉欧文氏菌,与噬菌体同时被检测到,结果证明其对病毒感染具有抗性。目前很难解释这一特性,不过有人注意到,在实验室条件下,欧文氏菌发生解离、溶原化和诱变时,其对噬菌体的抗性可能会发生巨大变化。噬菌体群体研究表明,它们是异质的,显然不仅可能包括多价噬菌体,还包括特异性病毒。对分离出的噬菌体纯系进行生物学和分子遗传学的进一步研究,将有助于更深入地了解噬菌体在细菌病原体与植物之间复杂关系网络中的地位和作用。

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