Duan Xiaolu, Zhang Liang, Zhou Daming, Ji Kaihua, Ma Ting, Shui Wenqing, Li Guoqiang, Li Xin
State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):597-601. doi: 10.1107/S1744309113011172. Epub 2013 May 23.
Dibenzothiophene (DBT) and its derivatives are typical sulfur compounds found in fossil fuels. These compounds show resistance to the hydrodesulfurization treatment that is commonly used in industry. Dibenzothiophene monooxygenase (DszC) is responsible for the oxidation of DBT, which is the first and the rate-limiting step in the DBT enzymatic desulfurization 4S pathway. In this study, the crystal structure of DszC from Rhodococcus erythropolis DS-3 is reported. The crystal of native DszC belonged to space group P1, with unit-cell parameters a = 96.16, b = 96.27, c = 98.56 Å, α = 81.03, β = 67.57, γ = 85.84°. To determine the phase, SAD X-ray diffraction data were collected from a SeMet-derivative DszC crystal, which also belonged to space group P1, with unit-cell parameters a = 95.379, b = 95.167, c = 94.891 Å, α = 87.046, β = 70.536, γ = 79.738°. Further structural analysis of DszC is in progress.
二苯并噻吩（DBT）及其衍生物是化石燃料中常见的典型含硫化合物。这些化合物对工业上常用的加氢脱硫处理具有抗性。二苯并噻吩单加氧酶（DszC）负责DBT的氧化，这是DBT酶促脱硫4S途径中的第一步且是限速步骤。在本研究中，报道了来自红平红球菌DS-3的DszC的晶体结构。天然DszC的晶体属于空间群P1，晶胞参数为a = 96.16，b = 96.27，c = 98.56 Å，α = 81.03，β = 67.57，γ = 85.84°。为了确定相位，从SeMet衍生的DszC晶体收集了SAD X射线衍射数据，该晶体也属于空间群P1，晶胞参数为a = 95.379，b = 95.167，c = 94.891 Å，α = 87.046，β = 70.536，γ = 79.738°。DszC的进一步结构分析正在进行中。
