异质成核有助于寻找地衣芽孢杆菌γ-谷氨酰胺转肽酶的初始结晶条件。
Heterogeneous nucleation helps the search for initial crystallization conditions of γ-glutamyl transpeptidase from Bacillus licheniformis.
作者信息
Lin Long Liu, Merlino Antonello
机构信息
Department of Applied Chemistry, National Chiayi University, 300 Syuefu Road, Chiayi City 60004, Taiwan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jun;69(Pt 6):669-72. doi: 10.1107/S1744309113012165. Epub 2013 May 25.
Abstract
Here, the crystallization and preliminary X-ray diffraction studies of Bacillus licheniformis γ-glutamyl transpeptidase (BlGT) are reported. The serendipitous finding of heterogeneous nucleants in the initial experiments provided the first crystallization conditions for the protein. Crystals were grown by hanging-drop vapour diffusion using a precipitant solution consisting of 20%(w/v) PEG 3350, 0.2 M magnesium chloride hexahydrate, 0.1 M Tris-HCl pH 8.2. The protein crystallized in the orthorhombic space group P2(1)2(1)2(1), with one heterodimer per asymmetric unit and unit-cell parameters a = 60.90, b = 61.97, c = 148.24 Å. The BlGT crystals diffracted to 2.95 Å resolution.
摘要
本文报道了地衣芽孢杆菌γ-谷氨酰转肽酶（BlGT）的结晶及初步X射线衍射研究。在初始实验中意外发现的异质成核剂为该蛋白提供了首个结晶条件。采用由20%（w/v）聚乙二醇3350、0.2 M六水合氯化镁、0.1 M Tris-HCl（pH 8.2）组成的沉淀剂溶液，通过悬滴气相扩散法生长晶体。该蛋白在正交晶系空间群P2(1)2(1)2(1)中结晶，每个不对称单元有一个异二聚体，晶胞参数a = 60.90、b = 61.97、c = 148.24 Å。BlGT晶体的衍射分辨率达到2.95 Å。
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1
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2
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Extremophiles. 2013 Jan;17(1):29-41. doi: 10.1007/s00792-012-0490-8. Epub 2012 Oct 27.
3
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Biochim Biophys Acta. 2013 Jan;1834(1):149-57. doi: 10.1016/j.bbapap.2012.09.014. Epub 2012 Oct 2.
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