Shimoi Akihito, Hatakeyama Hirofumi, Miyoshi Seiji, Watanabe Mitsutoshi
Ina Research Inc., 2148-188 Nishiminowa, Ina, Nagano 399-4501, Japan.
J Toxicol Pathol. 2013 Mar;26(1):11-7. doi: 10.1293/tox.26.11. Epub 2013 Apr 22.
In various kinds of glomerulonephritis, alteration of anionic charge on the glomerular basement membrane (GBM) and podocytes has been controversial for more than decade. To elucidate the relation between glomerular protein leakage and anionic sites on the glomerular wall, we examined the distribution of anionic sites on the GBM and podocytes of rats with active Heymann nephritis (AHN). Urinalysis for protein levels was conducted, and the kidneys were examined using electron microscopic cytochemistry for the assessment of anionic charge with two cationic probes. The anionic sites on podocytes were decreased in number in the AHN rats; however, the distributions of anionic sites on the GBM were similar in density to those seen in the control animals. From these results, we consider that the decrease in anionic charge density on podocytes might be attributable to protein leakage and that the charge barrier of the GBM is irrelevant to the protein leakage in AHN rats.
在各类肾小球肾炎中，肾小球基底膜（GBM）和足细胞上阴离子电荷的改变在过去十多年一直存在争议。为阐明肾小球蛋白渗漏与肾小球壁上阴离子位点之间的关系，我们检测了活动性海曼肾炎（AHN）大鼠GBM和足细胞上阴离子位点的分布。进行了尿蛋白水平分析，并使用电子显微镜细胞化学技术，用两种阳离子探针检测肾脏以评估阴离子电荷。AHN大鼠足细胞上的阴离子位点数量减少；然而，GBM上阴离子位点的分布密度与对照动物相似。根据这些结果，我们认为足细胞上阴离子电荷密度的降低可能归因于蛋白渗漏，并且GBM的电荷屏障与AHN大鼠的蛋白渗漏无关。
