Zhang Y P, Shi L M
Kunming Institute of Zoology, Academia Sinica, Yunnan.
Yi Chuan Xue Bao. 1990;17(1):23-33.
mtDNA from thirteen monkeys of five species (Macaca mulatta, M. nemestrina, M. assamensis, M. thibetana, M. arctoides) of the genus Macaca was analyzed with ten restriction enzymes, and compared with that of Japanese monkey (Macaca fuscata). Eight restriction types were observed among thirteen samples. There was extensive polymorphism in M. mulatta. The estimated number of nucleotide substitutions per site in M. mulatta is 0.012, and between these six species ranges from 0.016 to 0.091. Molecular phylogenetic tree of the mtDNA was constructed based on the genetic distance (P). The six species were divided into four groups: M nemestrina, M. arctoides, M. assamensis and M. thebitana, M. mulatta and M. fuscata. Our results support Fooden's (1976) classification of the genus Macaca into four species groups on the basis of morphologic data. Divergence times of the six species of the genus Macaca were also estimated on the mean rate of sequences divergence of 0.02 per million years in mtDNA.
对猕猴属5个物种（恒河猴、明打威猕猴、阿萨姆猕猴、藏酋猴、熊猴）的13只猴子的线粒体DNA（mtDNA）用10种限制性内切酶进行了分析，并与日本猴（食蟹猴）的mtDNA进行了比较。在13个样本中观察到8种限制性类型。恒河猴存在广泛的多态性。恒河猴每个位点的核苷酸替代估计数为0.012，这6个物种之间的核苷酸替代数在0.016至0.091之间。基于遗传距离（P）构建了mtDNA的分子系统发育树。这6个物种分为4组：明打威猕猴、熊猴、阿萨姆猕猴和藏酋猴，恒河猴和食蟹猴。我们的结果支持富登（1976年）根据形态学数据将猕猴属分为4个物种组的分类方法。还根据mtDNA每百万年0.02的序列平均分歧率估计了猕猴属6个物种的分歧时间。
