Su B, Wang W, Lan H, Zhang Y
Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences Kunming.
Yi Chuan Xue Bao. 1997 Apr;24(2):109-15.
In this paper, using protein electrophoresis method, we studied proteinpolymorphism and genetic divergence of 5 species in Genus Macaca: M. mulatta, M. arctoides, M. assamensis, M. thibetana, M. fascicularis. A total of 30 genetic loci were analyzed for 29 individuals, including 4 Nycticebus pygmaeus as outgroup. For the 19 M. mulatta, 9 loci were found to be polymorphic. Accordingly, the percentage of polymorphic loci, P = 0.3; the mean number of alleles, A = 1.4, and the mean heterozygosity, H = 0.1045, indicating a rather high level of genetic diversity in this species. Furthermore, 10 loci showed polymorphic among the 5 species, which can be used as information loci for phylogenetic reconstruction. Three programs (conml, neighbor, fitch) in PHYLIP 3.5 c were chosen to construct phylogenetic trees. All of the three trees show support a close relationship between M. mulatta and M. fascicularis. However, two trees have the same topology, suggesting that M. arctoides belongs to an independent species group, while M. assamensis and M. thibetana are closely related and belong to another species group, and the other tree gives a different topology which implies that M. arctoides, M. assamensis and M. thibetana belong to one species group.
在本文中，我们采用蛋白质电泳法研究了猕猴属5个物种的蛋白质多态性和遗传分化：恒河猴、熊猴、藏酋猴、藏猕猴、食蟹猴。对29个个体共分析了30个基因位点，包括4只倭蜂猴作为外群。对于19只恒河猴，发现9个位点具有多态性。相应地，多态位点百分比P = 0.3；平均等位基因数A = 1.4，平均杂合度H = 0.1045，表明该物种具有较高的遗传多样性水平。此外，在这5个物种中10个位点呈现多态性，可作为系统发育重建的信息位点。选用PHYLIP 3.5 c中的三个程序（conml、neighbor、fitch）构建系统发育树。这三棵树均显示恒河猴和食蟹猴之间关系密切。然而，有两棵树具有相同的拓扑结构，表明熊猴属于一个独立的物种组，而藏酋猴和藏猕猴关系密切，属于另一个物种组，另一棵树给出了不同的拓扑结构，这意味着熊猴、藏酋猴和藏猕猴属于一个物种组。
