Mondoulet Lucie, Dioszeghy Vincent, Ligouis Mélanie, Dhelft Véronique, Puteaux Emilie, Dupont Christophe, Benhamou Pierre-Henri
DBV Technologies, R&D Department, 92220 Bagneux, France.
ISRN Allergy. 2012 Feb 2;2012:375735. doi: 10.5402/2012/375735. Print 2012.
Background. The aim of this study was to compare the efficacy of epicutaneous immunotherapy (EPIT) to sublingual immunotherapy (SLIT) in a model of mice sensitized to Phleum pratense pollen. Methods. BALB/c mice were sensitized by sub-cutaneous route to pollen protein extract mixed treated for 8 weeks, using sham, EPIT, or SLIT. Measurements involved the serological response and cytokine profile from reactivated splenocytes, plethysmography after aerosol challenge to pollen, cell, and cytokine contents in the bronchoalveolar lavages (BALs). Results. After immunotherapy, sIgE was significantly decreased in the treated groups compared to sham (P < 0.001), whereas sIgG2a increased with EPIT and SLIT (P < 0.001 and P < 0.005 versus sham). Reactivated splenocytes secreted higher levels of Th2 cytokines with sham (P < 0.01). Penh values were higher in sham than EPIT and SLIT. Eosinophil recruitment in BAL was significantly reduced only by EPIT (P < 0.01). Conclusion. In this model of mice sensitized to pollen, EPIT was at least as efficient as SLIT.
背景。本研究的目的是在对早熟禾花粉致敏的小鼠模型中比较表皮免疫疗法（EPIT）与舌下免疫疗法（SLIT）的疗效。方法。将BALB/c小鼠通过皮下途径致敏于经处理的花粉蛋白提取物，持续8周，分别采用假处理、EPIT或SLIT。测量指标包括再激活脾细胞的血清学反应和细胞因子谱、花粉气溶胶激发后的体积描记法、支气管肺泡灌洗（BAL）中的细胞和细胞因子含量。结果。免疫治疗后，与假处理组相比，治疗组的sIgE显著降低（P < 0.001），而sIgG2a在EPIT和SLIT组中升高（与假处理组相比，P < 0.001和P < 0.005）。假处理组再激活的脾细胞分泌更高水平的Th2细胞因子（P < 0.01）。假处理组的Penh值高于EPIT和SLIT组。仅EPIT显著减少了BAL中嗜酸性粒细胞的募集（P < 0.01）。结论。在这个对花粉致敏的小鼠模型中，EPIT至少与SLIT一样有效。
