Haarder Simon, Jørgensen Kasper, Kania Per Walter, Skovgaard Alf, Buchmann Kurt
Laboratory of Aquatic Pathobiology, Section of Biomedicine, Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark.
Folia Parasitol (Praha). 2013 May;60(2):177-80. doi: 10.14411/fp.2013.020.
Freshwater pulmonate snails from three locations in Lake Furesø north of Copenhagen were screened for infection with furcocercariae (by shedding in the laboratory) and recovered parasite larvae were diagnosed by molecular methods (by performing PCR of rDNA and sequencing the internal transcribed spacer [ITS] region). Overall prevalence of infection in snails was 2%. Recovered cercariae from Lymnaea stagnalis (Linnaeus) were diagnosed as Diplostomum pseudospathaceum Niewiadomska, 1984 (prevalence 4%) and cercariae from Radix balthica (Linnaeus) as D. mergi (Dubois, 1932) (prevalence 2%). Pathogen-free rainbow trout were then exposed to isolated cercariae and infection success and site location of metacercariae in these fish were determined. Infection experiments confirmed that both species could infect rainbow trout with the eye lens as infection site for the metacercarial stage although infection success differed. Combination of molecular and biological assays may contribute to improvement of our knowledge on diagnosis, distribution and biology of diplostomids in fish.
对哥本哈根以北富勒瑟湖三个地点的淡水肺螺进行了尾蚴感染筛查（通过在实验室中让其排蚴），并通过分子方法（对核糖体DNA进行聚合酶链反应并对内部转录间隔区[ITS]进行测序）对回收的寄生虫幼虫进行诊断。蜗牛的总体感染率为2%。从静水椎实螺（林奈）中回收的尾蚴被诊断为伪斯氏双穴吸虫（Niewiadomska，1984）（感染率4%），而从椭圆萝卜螺（林奈）中回收的尾蚴被诊断为梅氏双穴吸虫（Dubois，1932）（感染率2%）。然后将无病原体的虹鳟鱼暴露于分离出的尾蚴中，并确定这些鱼体内后尾蚴的感染成功率和寄生部位。感染实验证实，这两种尾蚴均可感染虹鳟鱼，且后尾蚴阶段的感染部位均为晶状体，尽管感染成功率有所不同。分子和生物学检测方法的结合可能有助于增进我们对鱼类双穴吸虫的诊断、分布和生物学特性的了解。
