Okada Toshiya, Kataoka Yusuke, Takeshita Ai, Mino Masaki, Morioka Hiroshi, Kusakabe Ken Takeshi, Kondo Tomohiro
Department of Integrated Structural Biosciences, Division of Veterinary Science, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku Ourai Kita, Izumi-Sano, Osaka 598-8531, Japan.
Zoolog Sci. 2013 Jun;30(6):484-9. doi: 10.2108/zsj.30.484.
In the Mongolian gerbil, bilateral common carotid artery occlusion (BCCAO) for several minutes induces ischemia and delayed neuronal cell death in the CA1 region of the hippocampus due to their incomplete Circle of Willis. In the present study, the expression of fibroblast growth factor 2 (FGF2), its receptors (FGFR1 and FGFR2), glial fibrillary acidic protein (GFAP), and isolectin B4 (ISLB4) was investigated by immunohistochemical and lectin-binding methods after BCCAO was performed for 5 min in gerbils. One day after BCCAO, the pyramidal cells of the CA1 region of the hippocampus showed degenerative changes and lowered expression of FGF2, FGFR1, and FGFR2. Three days after BCCAO, there was an increase in GFAP-positive astrocytes and ISLB4-positive microglial cells. From five to 10 days after BCCAO, intense neuronal cell death in the stria pyramidale of the hippocampal CA1 region was observed, as well as an increase in GFAP-positive astrocytes and decrease in ISLB4-positive microglial cells. These results indicate that transient forebrain ischemia induces neuronal cell death with lowered expression of FGF2 and its receptors, and that the activation of glial cells may not directly lead to neuronal cell death.
在蒙古沙鼠中，由于其Willis环不完整，双侧颈总动脉闭塞（BCCAO）几分钟会诱发海马CA1区缺血和延迟性神经元细胞死亡。在本研究中，通过免疫组织化学和凝集素结合方法，对蒙古沙鼠进行5分钟BCCAO后，研究了成纤维细胞生长因子2（FGF2）、其受体（FGFR1和FGFR2）、胶质纤维酸性蛋白（GFAP）和异凝集素B4（ISLB4）的表达。BCCAO后一天，海马CA1区的锥体细胞出现退行性变化，FGF2、FGFR1和FGFR2的表达降低。BCCAO后三天，GFAP阳性星形胶质细胞和ISLB4阳性小胶质细胞增加。BCCAO后5至10天，观察到海马CA1区锥体层强烈的神经元细胞死亡，以及GFAP阳性星形胶质细胞增加和ISLB4阳性小胶质细胞减少。这些结果表明，短暂性前脑缺血会导致神经元细胞死亡，同时FGF2及其受体表达降低，并且胶质细胞的激活可能不会直接导致神经元细胞死亡。
