Watanabe H, Hastings J W
Department of Agricultural Biology Faculty of Agriculture, Kyoto University, Japan.
Biochim Biophys Acta. 1990 Jun 26;1017(3):229-34. doi: 10.1016/0005-2728(90)90189-b.
In bioluminescent bacteria very few agents have been reported that can selectively inhibit the luminescence. In sensitivity tests with Photobacterium phosphoreum, using 55 different antibiotics, it was found that sulfamethizole, an inhibitor of dihydropteroate synthetase and the formation of folic acid, inhibited bioluminescence more than growth. Likewise, in mutants requiring thymine for growth, the luminescence per cell was much less in a medium low in thymine. In neither case could the decreased specific luminescence be attributed to a decrease in the cellular level of luciferase or aldehyde factor; the involvement of additional but unidentified factors in the regulation of in vivo bioluminescence is postulated.
在发光细菌中，据报道很少有能选择性抑制发光的物质。在用55种不同抗生素对费氏弧菌进行敏感性测试时发现，二氢蝶酸合酶抑制剂和叶酸形成抑制剂磺胺甲噻二唑对生物发光的抑制作用大于对生长的抑制作用。同样，在需要胸腺嘧啶才能生长的突变体中，在胸腺嘧啶含量低的培养基中，每个细胞的发光要少得多。在这两种情况下，比发光的降低都不能归因于荧光素酶或醛因子细胞水平的降低；推测在体内生物发光调节中还涉及其他未确定的因素。
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