Kratasiuk V A, Fish A M
Biokhimiia. 1980 Jul;45(7):1175-81.
2,4-Dinitrofluorobenzene (DNFB) changes the parameters of the bioluminescent reaction involving two enzymes, i.e. NADH: FMN-oxidoreductase and luciferase, by decreasing the maximal intensity of luminescence and increasing the time of maximal intensity. Modification of the proteins does not affect the changes of the reaction parameters. The effects of DNFB on each one of the reactions, i.e. reduction of FNM and light emission, were studied. DNFB does not inhibit the reaction of FMN reduction. It was shown that the mechanism of DNFB effect of bioluminescence consists in uncoupling of FMN reduction and light emission due to competitive inhibition of the FMNH2 active center by 2,4-dinitrofluorobenzene.
2,4-二硝基氟苯（DNFB）通过降低发光的最大强度并延长达到最大强度的时间，改变了涉及两种酶（即NADH：FMN氧化还原酶和荧光素酶）的生物发光反应参数。蛋白质的修饰并不影响反应参数的变化。研究了DNFB对每个反应（即FMN的还原和发光）的影响。DNFB不抑制FMN的还原反应。结果表明，DNFB对生物发光的作用机制在于2,4-二硝基氟苯对FMNH2活性中心的竞争性抑制导致FMN还原与发光的解偶联。
