State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing, China.
Chin J Nat Med. 2013 May;11(3):222-30. doi: 10.1016/S1875-5364(13)60020-5.
To investigate the antioxidant and anti-inflammatory effects of Shengmai San (SMS) and its ethyl acetate extract (SEa), n-butanol extract (SBu), and aqueous extract (SWe), and clarify the material base of SMS and the roles played by its fractions.
A mouse model of transient forebrain ischemia/reperfusion (I/R) by means of common carotid artery occlusion (CCAO) was used to investigate the effects of SMS and its three fractions. Histopathological damage, blood-brain barrier disruption, and antioxidant and inflammation-related parameters, including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), myeloperoxidase (MPO), nitric oxide (NO), tumor necrosis factor-α (TNF-α) were measured. The chemical constituents of each fraction were identified by LC-MS.
Eighteen lignans in SEa, and thirteen steroidal glycosides and ginsenosides in SBu were determined. SMS significantly inhibited I/R induced formation of histological injury and cerebral MPO activity. SMS showed the strongest antioxidant and anti-inflammatory effects against the I/R-caused injuries. SEa showed higher antioxidant activity than the other two fractions and SBu has a slightly stronger inhibition on the productions of NO and TNF-α.
SMS as a whole had the most effective protection against cerebral I/R-caused injuries compared with its fractions, which inferred that it contains different groups of compounds that contribute together to its protective effect.
研究生脉散（SMS）及其乙酸乙酯提取物（SEa）、正丁醇提取物（SBu）和水提取物（SWe）的抗氧化和抗炎作用，阐明 SMS 的物质基础及其各馏分的作用。
采用颈总动脉结扎法（CCAO）制作短暂性全脑缺血/再灌注（I/R）小鼠模型，观察 SMS 及其三个馏分的作用。测量组织病理学损伤、血脑屏障破坏以及与氧化应激和炎症相关的参数，包括丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）、髓过氧化物酶（MPO）、一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）。采用 LC-MS 鉴定各馏分的化学成分。
SEa 中鉴定出 18 种木脂素，SBu 中鉴定出 13 种甾体糖苷和人参皂苷。SMS 显著抑制 I/R 引起的组织损伤和脑 MPO 活性。SMS 对 I/R 损伤具有最强的抗氧化和抗炎作用。SEa 显示出比其他两个馏分更高的抗氧化活性，而 SBu 对 NO 和 TNF-α的产生有稍微更强的抑制作用。
与各馏分相比，SMS 作为一个整体对脑 I/R 损伤具有最有效的保护作用，这表明它含有不同的化合物群，共同发挥其保护作用。
