Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.
J Chromatogr A. 2013 Jul 5;1297:196-203. doi: 10.1016/j.chroma.2013.04.078. Epub 2013 Apr 30.
Gas holdup time (tM) is a basic parameter in isothermal gas chromatography (GC). Determination and evaluation of tM and retention behaviors of n-alkanes under isothermal GC conditions have been extensively studied since the 1950s, but still remains unresolved. The difference equation (DE) model [J. Chromatogr. A 1260: 215-223] reveals retention behaviors of n-alkanes excluding tM, while the quadratic equation (QE) model [J. Chromatogr. A 1260: 224-231] including tM is suitable for applications. In the present study, tM values were calculated with the QE model, which is referred to as tMT, evaluated and compared with other three typical nonlinear models. The QE model gives an accurate estimation of tM in isothermal GC. The tMT values are highly accurate, stable, and easy to calculate and use. There is only one tMT value at each GC condition. The proper classification of tM values can clarify their disagreement and facilitate GC retention data standardization for which tMT values are promising reference tM values.
气体保留时间（tM）是等温气相色谱（GC）中的一个基本参数。自 20 世纪 50 年代以来，人们广泛研究了在等温 GC 条件下 tM 和正构烷烃保留行为的测定和评估，但仍未得到解决。差分方程（DE）模型[J. Chromatogr. A 1260: 215-223]揭示了除 tM 之外的正构烷烃的保留行为，而包括 tM 的二次方程（QE）模型[J. Chromatogr. A 1260: 224-231]适用于应用。在本研究中，使用 QE 模型（称为 tMT）计算 tM 值，并与其他三个典型的非线性模型进行评估和比较。QE 模型在等温 GC 中对 tM 进行了准确的估计。tMT 值非常准确、稳定，易于计算和使用。在每种 GC 条件下只有一个 tMT 值。对 tM 值进行适当的分类可以阐明它们之间的差异，并有助于 GC 保留数据的标准化，对于这一点，tMT 值是有前途的参考 tM 值。
