State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
Biomaterials. 2013 Sep;34(27):6377-87. doi: 10.1016/j.biomaterials.2013.05.005. Epub 2013 May 29.
A biodegradable in situ gel-forming controlled drug delivery system composed of curcumin loaded micelles and thermosensitive hydrogel was prepared and applied for cutaneous wound repair. Curcumin is believed to be a potent antioxidant and anti-inflammatory agent. Due to its high hydrophobicity, curcumin was encapsulated in polymeric micelles (Cur-M) with high drug loading and encapsulation efficiency. Cur-M loaded thermosensitive hydrogel (Cur-M-H) was prepared and applied as wound dressing to enhance the cutaneous wound healing. Cur-M-H was a free-flowing sol at ambient temperature and instantly converted into a non-flowing gel at body temperature. In vitro studies suggested that Cur-M-H exhibited well tissue adhesiveness and could release curcumin in an extended period. Furthermore, linear incision and full-thickness excision wound models were employed to evaluate the in vivo wound healing activity of Cur-M-H. In incision model, Cur-M-H-treated group showed higher tensile strength and thicker epidermis. In excision model, Cur-M-H group exhibited enhancement of wound closure. Besides, in both models, Cur-M-H-treated groups showed higher collagen content, better granulation, higher wound maturity, dramatic decrease in superoxide dismutase, and slight increase in catalase. Histopathologic examination also implied that Cur-M-H could enhance cutaneous wound repair. In conclusion, biodegradable Cur-M-H composite might have great application for wound healing.
一种由载姜黄素胶束和温敏水凝胶组成的可生物降解原位凝胶形成型控释药物传递系统被制备并应用于皮肤伤口修复。姜黄素被认为是一种有效的抗氧化剂和抗炎剂。由于其高疏水性，姜黄素被包裹在具有高载药量和包封效率的聚合物胶束（Cur-M）中。载姜黄素温敏水凝胶（Cur-M-H）被制备并用作伤口敷料，以增强皮肤伤口愈合。Cur-M-H 在环境温度下是自由流动的溶胶，在体温下立即转化为不流动的凝胶。体外研究表明，Cur-M-H 具有良好的组织粘附性，并能在延长的时间内释放姜黄素。此外，线性切口和全层切除伤口模型被用于评估 Cur-M-H 的体内伤口愈合活性。在切口模型中，Cur-M-H 处理组表现出更高的拉伸强度和更厚的表皮。在切除模型中，Cur-M-H 组表现出增强的伤口闭合。此外，在这两种模型中，Cur-M-H 处理组的胶原含量更高，肉芽组织更好，伤口成熟度更高，超氧化物歧化酶显著减少，过氧化氢酶略有增加。组织病理学检查也暗示 Cur-M-H 可以促进皮肤伤口修复。总之，可生物降解的 Cur-M-H 复合材料可能在伤口愈合方面有很大的应用前景。
