Departamento de Química Analítica, Facultade de Ciencias, Universidade da Coruña, Campus da Zapateira, E-15071 A Coruña, Spain.
J Chromatogr A. 2013 Jul 5;1297:226-30. doi: 10.1016/j.chroma.2013.05.020. Epub 2013 May 13.
The retention and separation of four homologues of benzalkonium chloride (alkyl (C12, C14, C16, C18) dimethylbenzylammonium chloride) have been studied in poly(dimethyldiphenylsiloxane) (TRB) and nitrile capillary phases, respectively. Under the optimized conditions (50% acetonitrile in processed samples, 35% of diphenyl content of the TRB, capillary length 43cm and water:methanol 60:40 as replacing solvent), the extraction efficiency was similar for all the homologues with satisfactory reproducibility and independently of the amount and proportion of homologues. Industrial samples with high viscosity or with complex composition and washes waters have been analyzed without previous treatment. The coupling of IT-SPME-CapLC-DAD to MS detection allowed the determination of the minority homologues (C16 and C18) in the industrial samples and washes waters. No matrix effect was found.
已分别在聚二甲基二苯基硅氧烷（TRB）和腈毛细管相上研究了 4 种苄索氯铵（烷基（C12、C14、C16、C18）二甲基苄基氯化铵）同系物的保留和分离。在优化条件下（处理样品中 50%的乙腈、TRB 的二苯基含量为 35%、毛细管长度为 43cm、水：甲醇 60：40 作为替代溶剂），所有同系物的萃取效率相似，重现性良好，且与同系物的数量和比例无关。无需预处理即可分析高粘度的工业样品或具有复杂组成和洗涤水的工业样品。IT-SPME-CapLC-DAD 与 MS 检测相结合，可在工业样品和洗涤水中测定少数同系物（C16 和 C18）。未发现基质效应。
