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低唾液酸化的α1-酸性糖蛋白抑制猫中性粒细胞的吞噬作用。

Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.

机构信息

Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, 20133 Milan, Italy.

出版信息

Res Vet Sci. 2013 Oct;95(2):465-71. doi: 10.1016/j.rvsc.2013.04.026. Epub 2013 May 31.

DOI:10.1016/j.rvsc.2013.04.026
PMID:23726663
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7126313/
Abstract

Feline α1-acid glycoprotein (fAGP) modifies both its serum concentration and its glycan moiety during diseases. fAGP is hyposialylated in cats with feline infectious peritonitis (FIP), but not in clinically healthy cats or in cats with other diseases. This study was aimed to determine whether hyposialylated fAGP influences phagocytosis. A flow cytometric method based on ingestion of fluoresceinated bacteria and adapted to feline blood was used to assess phagocytosis of leukocytes incubated with 'non-pathological' fAGP (purified from sera with normal concentrations of AGP) and 'pathological' fAGP (purified from sera with >1.5mg/mL hyposialylated AGP). The flow cytometric method provided repeatable results for neutrophils (coefficients of variations, CVs <15%) but not for monocytes (CVs>20%) which had also a high individual variability. Compared with saline solution and with non-pathological fAGP, pathological fAGP significantly decreased phagocytosis in neutrophils and monocytes. This study demonstrated that hyposialylated fAGP down-regulates the phagocytic activity of feline neutrophils.

摘要

猫α1-酸性糖蛋白（fAGP）在疾病期间会改变其血清浓度和聚糖部分。患有猫传染性腹膜炎（FIP）的猫的 fAGP 低唾液酸化，但在临床健康的猫或患有其他疾病的猫中则不会。本研究旨在确定低唾液酸化的 fAGP 是否会影响吞噬作用。本研究采用基于摄取荧光细菌的流式细胞术方法，并适应于猫血液，用于评估白细胞的吞噬作用，白细胞与“非病理”fAGP（从 AGP 浓度正常的血清中纯化）和“病理”fAGP（从 AGP 低唾液酸化> 1.5mg / mL 的血清中纯化）孵育。流式细胞术方法为中性粒细胞提供了可重复的结果（变异系数，CV<15％），但单核细胞则不然（CV> 20％），单核细胞的个体差异也很大。与生理盐水和非病理 fAGP 相比，病理 fAGP 显著降低了中性粒细胞和单核细胞的吞噬作用。这项研究表明，低唾液酸化的 fAGP 下调了猫中性粒细胞的吞噬活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/6f5f6d6024af/fx4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/8ef9c50741f0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/8e684fbf3b91/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/b67e8f49baf0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/3c4f1fbf21ef/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/f9f291fde91c/fx2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/fb4b3fb1d0b3/fx3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/6f5f6d6024af/fx4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/8ef9c50741f0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/8e684fbf3b91/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/b67e8f49baf0/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/3c4f1fbf21ef/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/f9f291fde91c/fx2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/fb4b3fb1d0b3/fx3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adf8/7126313/6f5f6d6024af/fx4.jpg

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