Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan; Institute of Life Sciences, National Defense Medical Center, Taipei 114, Taiwan.
Exp Neurol. 2013 Oct;248:10-5. doi: 10.1016/j.expneurol.2013.05.015. Epub 2013 May 28.
Visceral functions are regulated by a basal sympathetic nerve discharge (SND), also known as 'sympathetic tone'. We demonstrate herein that AC6 existed in tyrosine hydroxylase-positive rostral ventrolateral medulla neurons in the brainstem. Adenylyl cyclase (AC) assays showed lower basal and pituitary adenylate cyclase-activating peptide-evoked AC activities in the brainstem of AC6-null mice, indicating that AC6 is a prominent AC isozyme in the brainstem. Furthermore, two independent lines of AC6-null mice exhibited a much higher SND, recorded from splanchnic sympathetic nerves of neonatal brainstem-spinal cord preparations, than wildtype mice. An assay of urine noradrenaline confirmed this observation. Collectively, AC6 plays a critical role in the regulation of sympathetic tone.
内脏功能受基础交感神经放电（SND）调节，也称为“交感神经张力”。我们在此证明，AC6 存在于脑干中酪氨酸羟化酶阳性的头侧腹外侧延髓神经元中。腺苷酸环化酶（AC）测定显示，AC6 缺失小鼠的脑干中基础和垂体腺苷酸环化酶激活肽诱导的 AC 活性较低，表明 AC6 是脑干中主要的 AC 同工酶。此外，两条独立的 AC6 缺失小鼠表现出比野生型小鼠更高的 SND，该 SND是从新生脑-脊髓制备的内脏交感神经记录的。尿液去甲肾上腺素的测定证实了这一观察结果。总的来说，AC6 在调节交感神经张力方面起着关键作用。
