The First Clinical Medical School, Lanzhou University, Lanzhou 730000, China.
Peptides. 2013 Aug;46:33-9. doi: 10.1016/j.peptides.2013.05.011. Epub 2013 May 28.
Stearyl-cell penetrating peptides (CPPs) have been proved to be efficient nonviral gene vectors. Due to the similarities between antimicrobial peptides and CPPs, we constructed a novel type of gene vectors by introducing stearyl moiety to the N-terminus of antimicrobial peptide [D]-K6L9. In this study, stearyl-[D]-K6L9 delivered plasmids into cells by clathrin- and caveolin-mediated endocytosis. Gratifyingly, stearyl-[D]-K6L9 exhibited high transfection efficiency and almost reached the level of Lipofectamine 2000. Taken together, the combination of the stearyl moiety with [D]-K6L9 provides a novel framework for the development of excellent nonviral gene vectors.
硬脂酰-细胞穿透肽（CPPs）已被证明是有效的非病毒基因载体。由于抗菌肽和 CPPs 之间存在相似性，我们通过在抗菌肽 [D]-K6L9 的 N 端引入硬脂酰部分构建了一种新型基因载体。在这项研究中，硬脂酰-[D]-K6L9 通过网格蛋白和小窝蛋白介导的内吞作用将质粒递送到细胞中。令人高兴的是，硬脂酰-[D]-K6L9 表现出了很高的转染效率，几乎达到了 Lipofectamine 2000 的水平。综上所述，硬脂酰部分与 [D]-K6L9 的结合为开发优秀的非病毒基因载体提供了一个新的框架。
