Department of Clinical Laboratory Sciences, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia.
Food Chem Toxicol. 2013 Aug;58:530-5. doi: 10.1016/j.fct.2013.04.054. Epub 2013 May 30.
This study was designed to investigate the effect of butylated hydroxy toluene (BHT), a phenolic antioxidant used in foods, cosmetics and pharmaceutical products, on Fe-NTA-induced nephrotoxicity in rats. Fe-NTA (alone) treatment enhances ornithine decarboxylase activity to 5.3-fold, and [(3)H] thymidine incorporation in DNA to 3.5-fold compared with the corresponding saline treated control. The enhanced ornithine decarboxylase activity and DNA synthesis showed a reduction to 2.12-2.15-fold respectively at a higher dose of 2 mg BHT/day/animal, compared with the Fe-NTA treated group. Fe-NTA treatment also enhanced the renal microsomal lipid peroxidation to 2.0-fold and decreased the activities of glutathione and antioxidant enzymes to a range of 2.2-2.5-fold in kidney. These changes were reversed significantly in animals receiving a pretreatment of BHT. Present data suggests that BHT can prevent the toxic effects of Fe-NTA and can serve as a potent chemopreventive agent to suppress oxidant-induced tissue injury and nephrotoxicity in rats.
本研究旨在探讨丁基羟基甲苯 (BHT) 对 Fe-NTA 诱导的大鼠肾毒性的影响。BHT 是一种酚类抗氧化剂，用于食品、化妆品和医药产品中。Fe-NTA（单独）处理使鸟氨酸脱羧酶活性增强 5.3 倍，DNA 中 [(3)H] 胸腺嘧啶掺入增加 3.5 倍，与相应的生理盐水处理对照组相比。与 Fe-NTA 处理组相比，高剂量 2mg BHT/天/动物时，增强的鸟氨酸脱羧酶活性和 DNA 合成分别降低至 2.12-2.15 倍。Fe-NTA 处理还使肾微粒体脂质过氧化增强 2 倍，并使肾脏中的谷胱甘肽和抗氧化酶活性降低到 2.2-2.5 倍的范围内。这些变化在接受 BHT 预处理的动物中明显逆转。目前的数据表明，BHT 可以预防 Fe-NTA 的毒性作用，并可以作为一种有效的化学预防剂，抑制氧化剂诱导的组织损伤和大鼠的肾毒性。
