Bertram J F, Messina A, Ryan G B
Department of Anatomy, University of Melbourne, Parkville, Victoria, Australia.
Cell Tissue Res. 1990 May;260(3):555-63. doi: 10.1007/BF00297236.
Puromycin aminonucleoside (PAN)-induced nephrosis in rats provides a model for studying the pathogenesis of severe proteinuric conditions, such as minimal change disease. The present study used scanning (SEM) and transmission (TEM) electron microscopy to investigate the in vitro effects of PAN on rat glomerular podocytes. Slices of rat kidney were incubated for up to 3 days in Medium 199 with Hanks' salts (control) or in medium with PAN. Semiquantitative SEM analysis of glomeruli on the upper surface of kidney slices indicated that incubation with PAN (100 micrograms/ml and 500 micrograms/ml) decreased the number of microvilli on podocyte cell bodies (days 1, 2 and 3), increased the number of glomeruli showing flattening of podocyte cell bodies and major processes (days 2 and 3), and increased the number of glomeruli showing surface membrane blebbing on podocyte foot processes (day 3) (p less than 0.001 in all cases). TEM morphometry revealed that incubation with 500 micrograms/ml PAN retarded significantly (p less than 0.001 at days 2 and 3) the loss of podocyte foot processes observed in control cultures. Whilst the SEM changes to podocyte ultrastructure largely mimic those seen in PAN nephrosis in vivo, the retardation of foot process loss runs counter to the major TEM change observed in vivo.
嘌呤霉素氨基核苷（PAN）诱导的大鼠肾病为研究严重蛋白尿病症（如微小病变病）的发病机制提供了一个模型。本研究使用扫描电子显微镜（SEM）和透射电子显微镜（TEM）来研究PAN对大鼠肾小球足细胞的体外作用。将大鼠肾切片在含汉克斯盐的199培养基（对照）中或含PAN的培养基中孵育长达3天。对肾切片上表面的肾小球进行半定量SEM分析表明，用PAN（100微克/毫升和500微克/毫升）孵育会减少足细胞胞体上的微绒毛数量（第1、2和3天），增加显示足细胞胞体和主要突起扁平化的肾小球数量（第2和3天），并增加显示足细胞足突表面膜泡形成的肾小球数量（第3天）（所有情况下p均小于0.001）。TEM形态计量学显示，用500微克/毫升PAN孵育可显著延缓（第2和3天p小于0.001）对照培养物中观察到的足细胞足突丢失。虽然SEM观察到的足细胞超微结构变化在很大程度上模拟了体内PAN肾病中所见的变化，但足突丢失的延缓与体内观察到的主要TEM变化相反。
